LOCUS       sequence-70505-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLPe recombinase from the highly-expressed 
            CAGGS promoter; puro selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
  TITLE     Efficient method to generate single-copy transgenic mice by 
            site-specific integration in embryonic stem cells.
  JOURNAL   Genesis. 2006 Jan . 44(1):23-8.
  PUBMED    16400644
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2006 Jan . 44(1):23-8."
COMMENT     Sequence Label: pCAGGS-flpE-puro
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattacccg ccgccaccat ggctcccaag aagaagagga aggtgatgag
      121 tcaatttgat atattatgta aaacaccacc taaggtcctg gttcgtcagt ttgtggaaag
      181 gtttgaaaga ccttcagggg aaaaaatagc atcatgtgct gctgaactaa cctatttatg
      241 ttggatgatt actcataacg gaacagcaat caagagagcc acattcatga gctataatac
      301 tatcataagc aattcgctga gtttcgatat tgtcaacaaa tcactccagt ttaaatacaa
      361 gacgcaaaaa gcaacaattc tggaagcctc attaaagaaa ttaattcctg cttgggaatt
      421 tacaattatt ccttacaatg gacaaaaaca tcaatctgat atcactgata ttgtaagtag
      481 tttgcaatta cagttcgaat catcggaaga agcagataag ggaaatagcc acagtaaaaa
      541 aatgcttaaa gcacttctaa gtgagggtga aagcatctgg gagatcactg agaaaatact
      601 aaattcgttt gagtatacct cgagatttac aaaaacaaaa actttatacc aattcctctt
      661 cctagctact ttcatcaatt gtggaagatt cagcgatatt aagaacgttg atccgaaatc
      721 atttaaatta gtccaaaata agtatctggg agtaataatc cagtgtttag tgacagagac
      781 aaagacaagc gttagtaggc acatatactt ctttagcgca aggggtagga tcgatccact
      841 tgtatatttg gatgaatttt tgaggaattc tgaaccagtc ctaaaacgag taaataggac
      901 cggcaattct tcaagcaaca aacaggaata ccaattatta aaagataact tagtcagatc
      961 gtacaca
//
