LOCUS       sequence-70506-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLPe recombinase from the highly-expressed 
            CAGGS promoter; puro selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
  TITLE     Efficient method to generate single-copy transgenic mice by 
            site-specific integration in embryonic stem cells.
  JOURNAL   Genesis. 2006 Jan . 44(1):23-8.
  PUBMED    16400644
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2006 Jan . 44(1):23-8."
COMMENT     Sequence Label: pCAGGS-flpE-puro
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    11..218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     207..224
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(388..408)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg
       61 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
      121 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
      181 ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc agcgagctct
      241 agatcatcga tgcatggggt cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg
      301 ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
      361 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
      421 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
      481 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
      541 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
      601 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
      661 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
      721 cgatgtcgag cccgacgcgc gtgag
//
