LOCUS       sequence-70510-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector containing MEKDD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-MEKDD
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaag acgccagcag catgggttgg
      121 tgtgctgggc tggttaaggc cgatggtgga gcagagccaa cctgcaaaat ccacttcctc
      181 agcatcagat ctcttgataa aagcatgaac catgagttgc ttcaaatctg ctctctctgc
      241 ggggtttttt attaagcatt tattcacaaa atcttgaaat tccagactga acactccact
      301 gggcagtttt ggaggaggct cgttgactat gtaatccaac aactcaaaaa ttgccatggg
      361 aggtcggctg tccattccgt atgagctaag gggcctcccg ggggtccttg gcctgggtgg
      421 ggtctcagcc gcatctcctt ccacctggca cccaaacatc agctccagct ccttggcatc
      481 tggaggaggg atgggatacc tcccaaccgc catctctacc agagacagtc ccatgctcca
      541 gatgtctgac tgcacagagt aatgagtccc ctggagtctt tctggcgaca tgtaggacct
      601 tgtgcccacg aagtcgttgg ccatgtcgtc gatgagctgc ccgctgaccc caaagtcaca
      661 gagcttgatc tccccacggg agttgactag gatgttggag ggcttgacat ctctgtgcat
      721 gatcttgtgc ttctccctca gatatgtcag gccttttatt acagcaatgc taacttttcc
      781 taaaatttgt tcaggaattc ttccagcttt cttcaggact tgatcca
//
