LOCUS       sequence-70511-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian cell codon-optimized dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pMLM3705
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg
       61 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt
      121 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac
      181 cgtttatctg attacgacgt cgatgccatt gtaccccaat cctttttgaa ggacgattca
      241 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca
      301 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg
      361 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt
      421 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt
      481 gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg
      541 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa
      601 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc
      661 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt
      721 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag
      781 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact
      841 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccc
//
