LOCUS       sequence-70519-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human Dendrin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, Sumita K, 
            Fujiwara N, Rokukawa C, Hamano M, Hirabayashi S, Hata Y
  TITLE     CIN85 is localized at synapses and forms a complex with S-SCAM via 
            dendrin.
  JOURNAL   J Biochem. 2006 May;139(5):931-9.
  PUBMED    16751601
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biochem."; date: "2006-05"; volume: "139(5)"; pages: "931-9"
COMMENT     Sequence Label: pClneoFLAG human Dendrin
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          168..300
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     283..302
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        345..363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             378..401
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctcg tttagtgacc gtcagatcac tagaagcttt attgcggtag tttatcacag
       61 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
      121 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      181 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      241 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      301 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
      361 aggctagccg ggccatggac tacaaagacg atgacgacaa ggaattcctg gatggcccac
      421 tgttctccga ggggcctgac agcccccggg agctccagga tgaggagtct ggcagctgcc
      481 tctgggtgca gaagtccaag ctattggtga tagaagtgaa gactatttcc tgtcattata
      541 gtcgccgcgc cccttctcga cagcccatgg acttccaggc cagccactgg gctcgcgggt
      601 tccagaaccg cacgtgtggg ccgcgcccgg gatccccaca gccgccgccc cgccggccct
      661 gggcctccag ggtgctgcag gaggcgacca actggcgggc ggggcccctg gccgaggtcc
      721 gagctcggga gcaagagaaa aggaaagcgg cgtcgcagga gcgggaggcc aaggagaccg
      781 agcgaaaaag gcgcaaggct ggtggggccc gacggagccc cccgggtcga ccccgcccgg
      841 agccccgcaa cgcccctcgg gtggcccagc tggcagggct ccctgctccc ttgcggccgg
      901 agcgcctggc gcctgtgggg cgagcgcccc gtccatccgc gcagccgcag a
//
