LOCUS       sequence-70521-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Myc tagged human CIN85 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, Sumita K, 
            Fujiwara N, Rokukawa C, Hamano M, Hirabayashi S, Hata Y
  TITLE     CIN85 is localized at synapses and forms a complex with S-SCAM via 
            dendrin.
  JOURNAL   J Biochem. 2006 May;139(5):931-9.
  PUBMED    16751601
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biochem."; date: "2006-05"; volume: "139(5)"; pages: "931-9"
COMMENT     Sequence Label: pClneoMyc human CIN85
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          170..302
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     285..304
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        347..365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             386..415
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      121 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      181 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      241 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      301 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
      361 ataggctagc agacccccca acatggagca gaagcttatc agcgaggagg acctgctcga
      421 ggaattcgtg gaggccatag tggagtttga ctaccaggcc cagcacgatg atgagctgac
      481 gatcagcgtg ggtgaaatca tcaccaacat caggaaggag gatggaggct ggtgggaggg
      541 acagatcaac ggcaggagag gtttgttccc tgacaacttt gtaagagaaa taaagaaaga
      601 gatgaagaaa gaccctctca ccaacaaagc tccagaaaag cccctgcacg aagtgcccag
      661 tggaaactct ttgctgtctt ctgaaacgat tttaagaacc aataagagag gcgagcgacg
      721 gaggcgccgg tgccaggtgg cattcagcta cctgccccag aatggcgatg aacttgagct
      781 gaaagttggc gacatcatag aggtggtagg agaggtagag gaaggatggt gggaaggtgt
      841 tctcaacggg aagactggaa tgtttccttc caacttcatc aaggagctgt caggggagtc
      901 ggatgagctt ggcatttccc aggatgagca gctatccaag tcaagtttaa gg
//
