LOCUS       sequence-70524-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Myc tagged human MOB1 containing T12A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Bao Y, Sumita K, Kudo T, Withanage K, Nakagawa K, Ikeda M, Ohno K, 
            Wang Y, Hata Y
  TITLE     Roles of mammalian sterile 20-like kinase 2-dependent 
            phosphorylations of Mps one binder 1B in the activation of nuclear 
            Dbf2-related kinases.
  JOURNAL   Genes Cells. 2009 Dec;14(12):1369-81. doi: 
            10.1111/j.1365-2443.2009.01354.x. Epub 2009 Nov 16.
  PUBMED    19919647
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2443.2009.01354.x"; journalName: "Genes Cells"; 
            date: "2009-12"; volume: "14"; issue: "12"; pages: "1369-81"
COMMENT     Sequence Label: pClneoMyc MOB1 T12A
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             367..396
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag cagacccccc
      361 aacatggagc agaagcttat cagcgaggag gacctgctcg aggaattcac gcgtatgagc
      421 ttcctcttca gcagccgctc ttctaaagca ttcaaaccaa agaagaatat ccctgaagga
      481 tctcatcagt atgaactctt aaaacatgca gaagcaactc taggaagtgg gaatctgaga
      541 caagctgtta tgttgcctga gggagaggat ctcaatgaat ggattgctgt gaacactgtg
      601 gatttcttta accagatcaa catgttatat ggaactatta cagaattctg cactgaagca
      661 agctgtccag tcatgtctgc aggtccgaga tatgaatatc actgggcaga tggtactaat
      721 attaaaaagc caatcaaatg ttctgcacca aaatacattg actatttgat gacttgggtt
      781 caagatcagc ttgatgatga aactcttttt ccttctaaga ttggtgtccc atttcccaaa
      841 aactttatgt ctgtggcaaa gaccattcta aagcgtctgt tcagggttta tgcccatatt
      901 tatcaccagc actttgattc tgtgatgcag ctgcaagagg aggcccacct caacacctcc
      961 tttaagcact ttattttctt tgttc
//
