LOCUS       sequence-70527-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Myc tagged human MOB1 containing T12A and T35A mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Bao Y, Sumita K, Kudo T, Withanage K, Nakagawa K, Ikeda M, Ohno K, 
            Wang Y, Hata Y
  TITLE     Roles of mammalian sterile 20-like kinase 2-dependent 
            phosphorylations of Mps one binder 1B in the activation of nuclear 
            Dbf2-related kinases.
  JOURNAL   Genes Cells. 2009 Dec;14(12):1369-81. doi: 
            10.1111/j.1365-2443.2009.01354.x. Epub 2009 Nov 16.
  PUBMED    19919647
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2443.2009.01354.x"; journalName: "Genes Cells"; 
            date: "2009-12"; volume: "14"; issue: "12"; pages: "1369-81"
COMMENT     Sequence Label: pClneoMyc MOB1 T12/35A
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          152..284
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     267..286
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        329..347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             368..397
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat tgctaacgca
       61 gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gtgactctct taaggtagcc
      121 ttgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      181 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      241 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
      301 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcagaccccc
      361 caacatggag cagaagctta tcagcgagga ggacctgctc gaggaattca cgcgtatgag
      421 cttcctcttc agcagccgct cttctaaagc attcaaacca aagaagaata tccctgaagg
      481 atctcatcag tatgaactct taaaacatgc agaagcagct ctaggaagtg ggaatctgag
      541 acaagctgtt atgttgcctg agggagagga tctcaatgaa tggattgctg tgaacactgt
      601 ggatttcttt aaccagatca acatgttata tggaactatt acagaattct gcactgaagc
      661 aagctgtcca gtcatgtctg caggtccgag atatgaatat cactgggcag atggtactaa
      721 tattaaaaag ccaatcaaat gttctgcacc aaaatacatt gactatttga tgacttgggt
      781 tcaagatcag cttgatgatg aaactctttt tccttctaag attggtgtcc catttcccaa
      841 aaactttatg tctgtggcaa agactattct aaagcgtctg ttc
//
