LOCUS       sequence-70528-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: 7TFC
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        255..286
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      342..351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(414..432)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag cctcctagcc
       61 cgggctcgag cagctgaagc ttgcatgcct gcagggtacc gagctcttac gcgagatcaa
      121 agggggtaag atcaaagggg gtaagatcaa aggggcgcga gatcaaaggg ggtaagatca
      181 aagggggtaa gatcaaaggg ggtaagatca aaggggcgcg cccgcgtgct agcccgggct
      241 cgagatctag actctagagg gtatataatg gaagctcgaa ttccagcttg gcattccggt
      301 actgttggta aaaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca
      361 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
      421 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
      481 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
      541 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
      601 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
      661 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
      721 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
      781 tcatccaaaa aattattatc
//
