LOCUS       sequence-70530-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Myc tagged human MOB1 containing T74A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bao Y, Sumita K, Kudo T, Withanage K, Nakagawa K, Ikeda M, Ohno K, 
            Wang Y, Hata Y
  TITLE     Roles of mammalian sterile 20-like kinase 2-dependent 
            phosphorylations of Mps one binder 1B in the activation of nuclear 
            Dbf2-related kinases.
  JOURNAL   Genes Cells. 2009 Dec;14(12):1369-81. doi: 
            10.1111/j.1365-2443.2009.01354.x. Epub 2009 Nov 16.
  PUBMED    19919647
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2443.2009.01354.x"; journalName: "Genes Cells"; 
            date: "2009-12"; volume: "14"; issue: "12"; pages: "1369-81"
COMMENT     Sequence Label: pClneoMyc MOB1 T74A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          149..281
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     264..283
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        326..344
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             365..394
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc taacgcagtc
       61 agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa ggtagccttg
      121 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
      181 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      241 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
      301 ttacagctct taaggctaga gtacttaata cgactcacta taggctagca gaccccccaa
      361 catggagcag aagcttatca gcgaggagga cctgctcgag gaattcacgc gtatgagctt
      421 cctcttcagc agccgctctt ctaaaacatt caaaccaaag aagaatatcc ctgaaggatc
      481 tcatcagtat gaactcttaa aacatgcaga agcaactcta ggaagtggga atctgagaca
      541 agctgttatg ttgcctgagg gagaggatct caatgaatgg attgctgtga acactgtgga
      601 tttctttaac cagatcaaca tgttatatgg agctattaca gaattctgca ctgaagcaag
      661 ctgtccagtc atgtctgcag gtccgagata tgaatatcac tgggcagatg gtactaatat
      721 taaaaagcca atcaaatgtt ctgcaccaaa atacattgac tatttgatga cttgggttca
      781 agatcagctt gatgatgaaa ctctttttcc ttctaagatt ggtgtcccat ttcccaaaaa
      841 ctttatgtct gtggcaaaga ctattctaaa gcgtctgttc
//
