LOCUS       sequence-70542-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  control FRET sensor for beta2-adrenergic receptor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, 
            Sivaramakrishnan S
  TITLE     Detection of G Protein-selective G Protein-coupled Receptor (GPCR) 
            Conformations in Live Cells.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 
            10.1074/jbc.M113.464065. Epub 2013 Apr 29.
  PUBMED    23629648
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464065"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17167-78"
COMMENT     Sequence Label: pCDNA_B2AR-no-pep
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(77..787,788..790))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(119..140)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     468..487
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     727..748
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1046..1066)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aacgggccct ctagactcga gcggccgctc atccgcttcc tccgcttcct ccgcttcctc
       61 cgcttccgtt aattaacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      121 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact
      181 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      241 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      301 tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtcg ctgatggcgt
      361 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
      421 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
      481 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
      541 agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg ccccaggtca
      601 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
      661 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
      721 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
      781 ccttgctcac gggcgcgccg ccagagccgc cagagccctt cttcttgcgt ttttcatcat
      841 cttccctttt ctttctctct tcttcttctt gttttctctt tgcttccatc tcaagtttca
      901 tccgcctttc ctcctcctcc tttcttcgac gttgttcatc ttcttcacgt ctttttcttt
      961 ccttttccat ttcttcttga atacgcctca gcctttctgc ttcctcttcc tgctgttttt
     1021 ttttctcttc ctcttctccg cttccgccct gaaaatacag gttttctccg cttcctccgc
     1081 ttccgaattc cttgta
//
