LOCUS       sequence-70549-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET sensor for beta2-adrenergic receptor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, 
            Sivaramakrishnan S
  TITLE     Detection of G Protein-selective G Protein-coupled Receptor (GPCR) 
            Conformations in Live Cells.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 
            10.1074/jbc.M113.464065. Epub 2013 Apr 29.
  PUBMED    23629648
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464065"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17167-78"
COMMENT     Sequence Label: pCDNA_B2AR-q-pep
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(149..859,860..862))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(191..212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     540..559
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     799..820
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgggccct ctagactcga gcggccgctt agaccagatt gtactccttc aggttcaact
       61 ggaggatggt gtccttgacg gcagcaaaga caaagcggat attctcggtg tctccacttc
      121 cacctgaccc gccgcttccg ttaattaact tgtacagctc gtccatgccg agagtgatcc
      181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      241 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      361 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      421 cgctgatggc gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      481 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      541 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      601 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca
      661 cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      721 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      781 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      841 acagctcctc gcccttgctc acgggcgcgc cgccagagcc gccagagccc ttcttcttgc
      901 gtttttcatc atcttccctt ttctttctct cttcttcttc ttgttttctc tttgcttcca
      961 tctcaagttt catccgcctt tcctcctcct cctttcttcg acgttgttca tcttcttcac
     1021 gtctttttct ttccttttcc atttcttctt gaatacg
//
