LOCUS       sequence-70554-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET sensor for beta2-adrenergic receptor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, 
            Sivaramakrishnan S
  TITLE     Detection of G Protein-selective G Protein-coupled Receptor (GPCR) 
            Conformations in Live Cells.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 
            10.1074/jbc.M113.464065. Epub 2013 Apr 29.
  PUBMED    23629648
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464065"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17167-78"
COMMENT     Sequence Label: pCDNA_B2AR-s-pep
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(161..871,872..874))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(203..224)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     552..571
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     811..832
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgggccct ctagactcga gcggccgcta cactgagctc tcagagcagc tcgtactgac
       61 gaaggtgcat gcgctgaatg atgtcacggc agtcgttgaa cacacggcgg atgttctcag
      121 tgtctccact tccacctgac ccgccgcttc cgttaattaa cttgtacagc tcgtccatgc
      181 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      241 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      301 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      361 cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga
      421 tatagacgtt gtcgctgatg gcgttgtact ccagcttgtg ccccaggatg ttgccgtcct
      481 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      541 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      601 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
      661 cgaagcactg cacgccccag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      721 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      781 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      841 ccaccccggt gaacagctcc tcgcccttgc tcacgggcgc gccgccagag ccgccagagc
      901 ccttcttctt gcgtttttca tcatcttccc ttttctttct ctcttcttct tcttgttttc
      961 tctttgcttc catctcaagt ttcatccgcc tttcctcctc ctcctttctt cgacgttgtt
     1021 ca
//
