LOCUS       sequence-70556-e        8222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7a
FEATURES             Location/Qualifiers
     source          1..8222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2..796)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2..796)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(113..132)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     723..742
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    1205..1326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1368..1388
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1442..2041)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1525..1545)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2022..2041
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2042..2095)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2165..2827)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      2848..2857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             2886..3154
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3167..3378)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     rep_origin      3599..4187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4088..4107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4284..4663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4665..4942
                     /label=chicken beta-actin promoter
     intron          4943..5960
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5884..5906
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             complement(5969..6790)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQQKLEDLEGWPVTQFAARSLAVVLQRRDWENPGVTQL
                     NRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGGSCQSIPPSVHSFH
                     SCHSAAFSDLQQLSSVQSGLEWWTPQFPGDGEECFECSGTAPLLSDAHNGFVGCLRQSG
                     DHVLIFDGHSPECSGMLRQSHNMGVCETVPHQCFMVLSDGGPHLWFDLLLLLLAVAEGS
                     QIHLGCWTGGYPTSATTSATWFIVVILVVSGRHLPLFLRSHGGGPYSESYY"
     primer_bind     5973..5992
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5973..5991
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5996..6005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6008..6028
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6038..6061
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     6679..6701
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6693..6710
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6694..6710
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6786..6802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6786..6802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6799..6821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6810..6826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6834..6840,6841..6858,6859..6864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6879..6900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7077..7664
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(7685..7701)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7685..7701)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          7861..7926
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    7973..8107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
       61 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
      121 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
      181 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
      241 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg
      301 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
      361 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
      421 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
      481 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
      541 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      601 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      661 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      721 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      781 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
      841 tgcgccatca gatccttggc ggcgagaaag ccatccagtt tactttgcag ggcttcccaa
      901 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
      961 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     1021 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     1081 gactggcttt ctacgtgaaa aggatctagg tgaagatcct ttttgataat ctcatgaccc
     1141 ggaagggaga ccagatagct tctgatagac taattgacat catttgagtc aattccagac
     1201 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1261 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1321 caagtttagt taacgcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     1381 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     1441 ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
     1501 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
     1561 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
     1621 tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg
     1681 gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg
     1741 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
     1801 tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga
     1861 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
     1921 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
     1981 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
     2041 tagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg ccctcgcgag
     2101 atccggtgga gccgggtccg gcggtgccgt ccacggcaga attggacgac tgagcgcggg
     2161 atctggcgaa ggcgatgggg gtcttgaagg cgtgctggta ctccacgatg cccagctcgg
     2221 tgttgctgtg cagctcctcc acgcggcgga aggcgaacat ggggcccccg ttctgcagga
     2281 tgctggggtg gatggcgctc ttgaagtgca tgtggctgtc caccacgaag ctgtagtagc
     2341 cgccgtcgcg caggctgaag gtgcgggcga agctgcccac cagcacgtta tcgcccatgg
     2401 ggtgcaggtg ctccacggtg gcgttgctgc ggatgatctt gtcggtgaag atcacgctgt
     2461 cctcggggaa gccggtgccc accaccttga agtcgccgat cacgcggccg gcctcgtagc
     2521 ggtagctgaa gctcacgtgc agcacgccgc cgtcctcgta cttctcgatg cgggtgttgg
     2581 tgtagccgcc gttgttgatg gcgtgcagga aggggttctc gtagccgctg gggtaggtgc
     2641 cgaagtggta gaagccgtag cccatcacgt ggctcagcag gtaggggctg aaggtcaggg
     2701 cgcctttggt gctcttcatc ttgttggtca tgcggccctg cttgggggtg ccctctccgc
     2761 cgcccaccag ctcgaactcc acgccgttca gggtgccggt gatgcggcac tcgatctcca
     2821 tggcgggcag gccgctctcg tcgctctcca tggtggcgtc tagcgtaggc gccggtcaca
     2881 gcttggatct gtaacggcgc agaacagaaa acgaaacaaa gacgtagagt tgagcaagca
     2941 gggtcaggca aagcgtggag agccggctga gtctaggtag gctccaaggg agcgccggac
     3001 aaaggcccgg tctcgacctg agctttaaac ttacctagac ggcggacgca gttcaggagg
     3061 caccacaggc gggaggcggc agaacgcgac tcaaccggcg tggatggcgg cctcaggtag
     3121 ggcggcgggc gcgtgaagga gagatgcgag cccctcgaag cttcagctgt gttctggcgg
     3181 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     3241 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     3301 accttctcta ggcacccgtt caattgccga cccctccccc caacttctcg gggactgtgg
     3361 gcgatgtgcg ctctgcccac tgacgggcac cggagcgatc gcagatcctt cgcggccgcg
     3421 gatccgattt aaattcgaat tcgctagctc tagaatacgg gtatccaggg gacacagtct
     3481 atcagaggac tggcccacca agtgaggggt tgtgggctct tttcatgacc aaaatccctt
     3541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3721 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4201 cggccttttt acggttcctg ggcttttgct ggccttttgc tcacatgttc tttcctgcgt
     4261 tatcccctgg gcgcgccgga ttcgacattg attattgact agttattaat agtaatcaat
     4321 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4381 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4441 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4501 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4561 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4621 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     4681 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     4741 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg
     4801 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     4861 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4921 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     4981 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     5041 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     5101 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     5161 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     5221 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     5281 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     5341 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     5401 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     5461 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     5521 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     5581 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     5641 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     5701 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     5761 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     5821 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     5881 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     5941 ccttcttctt tttcctacag atccttaatt aataatacga ctcactatag gggccgccac
     6001 catgggacct aagaaaaaga ggaaggtggc ggccgctgac tacaaggatg acgacgataa
     6061 accaggtggc ggaggtagtg gcggaggtgg ggtacccgcc agtccagcag cccaggtgga
     6121 tctgagaacc ctcggctaca gccagcagca gcaggagaag atcaaaccaa aggtgcggtc
     6181 caccgtcgct cagcaccatg aagcactggt ggggcacggt ttcacacacg cccatattgt
     6241 ggctctgtct cagcatcccg ctgcactcgg gactgtggcc gtcaaatatc aggacatgat
     6301 cgccgctctg cctgaggcaa cccacgaagc cattgtgggc gtcggaaagc agtggagcgg
     6361 tgccagagca ctcgaagcac tcctcaccgt cgccggggaa ctgcggggtc caccactcca
     6421 gtccggactg gacactggac agctgctgaa gatcgctaaa cgcggcggag tgacagctgt
     6481 ggaagctgtg cacgcttgga ggaatgctct gacaggagcc cccctggaga cgggcgccgc
     6541 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6601 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6661 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgg
     6721 cgaattgggt aacgggccac ccctcgaggt cctccagctt ttgttgcgcg cttggcgtaa
     6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6901 attgcgttgc gctcactgcc cgctttccac cggtcgtctc caacgatcat ctggtcgctc
     6961 tcgcttgcct cggtggacgg cccgctctgg acgcagtcaa aaagggtctc ccccatgctc
     7021 ccgcactgat caagagaacc aacaggagaa ttcctgaggg atccgatcgt ttaaaccagc
     7081 tcgtgaaaag cgaactcgaa gaaaagaaaa gtgaactgcg gcacaaactg aaatacgtcc
     7141 cacatgaata cattgagctg atcgagattg ctaggaactc cacccaggac agaatcctcg
     7201 agatgaaagt gatggaattc tttatgaaag tctacgggta tcggggcaag cacctgggcg
     7261 gatctcgcaa accagatggg gcaatctaca ctgtgggtag tcccatcgac tatggcgtga
     7321 ttgtcgatac caaggcctac agtgggggtt ataatctgcc cattggacag gctgacgaga
     7381 tgcagcgata cgtggaggaa aaccagacaa gaaataagca tatcaacccc aatgagtggt
     7441 ggaaagtgta tcctagctcc gtcactgaat tcaagtttct cttcgtgtca ggccacttta
     7501 agggaaacta caaagcacag ctgaccaggc tcaatcatat tacaaactgc aatggcgccg
     7561 tgctgagcgt cgaggaactg ctcatcggcg gagagatgat caaggccggc acactcaccc
     7621 tggaggaggt ccgccgaaaa ttcaataacg gggaaatcaa cttctgaacg cgtaaatgat
     7681 tgcagatcca ctagttctag aattccagct gagcgccggt cgctaccatt accagttggt
     7741 ctggtgtcaa aaataataat aaccgggcag gggggatctg catggatctt tgtgaaggaa
     7801 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     7861 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     7921 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc cagatccaga
     7981 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     8041 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     8101 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     8161 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctgcggcc
     8221 gc
//
