LOCUS       sequence-7057-ex       14367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, 
            Schmidt-Supprian M
  TITLE     Canonical NF-kappaB activity, dispensable for B cell development, 
            replaces BAFF-receptor signals and promotes B cell proliferation 
            upon activation.
  JOURNAL   Immunity. 2006 Jun . 24(6):729-39.
  PUBMED    16782029
REFERENCE   2  (bases 1 to 14367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2006 Jun . 24(6):729-39."
COMMENT     Sequence Label: STOP-eGFP-ROSA26TV
FEATURES             Location/Qualifiers
     source          1..14367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..1086
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1093..1158,1159..1196)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    complement(1207..1240)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1247..1295
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1302..2105)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1413..1432)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2023..2042
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     intron          2919..2984
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3114..3134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3559..3693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3596..3615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3650..3669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3713..3746)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3779..3826
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    3865..4416
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4009..4026)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4236..4255
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4428..4430,4431..4433,4434..5147)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4428..4430,4431..4433,4434..5144)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4473..4494)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4734..4753)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5081..5102
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5172..5219
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(5244..5260)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5279..5503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5591..9848
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        9897..10387
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     10320..10339
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10414..10983
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          11060..11125
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    11199..11423
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11522..11542)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11522..11540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11561..11577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11561..11577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11574..11596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11585..11601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11609..11615,11616..11633,11634..11639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11654..11675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11792..11809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11963..12551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12043..12062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12722..13513,13514..13582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13345..13364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13583..13687)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13713..14168)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13850..13871)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14062..14081
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14295..14317
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14309..14326
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14310..14326
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14336..14355
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14336..14354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
       61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
      121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
      181 ggggggcagc ggaggacgct tccggccgac gtctcgtcgc tgattggctt ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctga cgtgcgggcg cggtcggcct ctggcggggc gggggagggg
      421 agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc cttcctctgg
      481 gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct cggggcccag
      541 aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg ccggccagcg
      601 ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg ccgcagagtc
      661 tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg gacgggcagt
      721 agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg cctcaagagg
      781 ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg agcgagggct
      841 cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc tgagttgtta
      901 tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct ccggaggggg
      961 gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct tctgaggacc
     1021 gccctgggcc tgggagaatc ccttgccccc tcttcccctc gtgatctgca actccagtct
     1081 ttctagcatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
     1141 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtggtt
     1201 aattaaataa cttcgtatag catacattat acgaagttat ggatccgaac aaacgaccca
     1261 acacccgtgc gttttattct gtctttttat tgccgatccc ctcagaagaa ctcgtcaaga
     1321 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     1381 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     1441 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     1501 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     1561 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     1621 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     1681 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     1741 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     1801 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     1861 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     1921 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     1981 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     2041 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat
     2101 cccatattgg ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga
     2161 agtggacctg ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa
     2221 gacgctgggc ggggtttgct cgacattggg tggaaacatt ccaggcctgg gtggagaggc
     2281 tttttgcttc ctcttgcaaa accacactgc tcgacattgg gtggaaacat tccaggcctg
     2341 ggtggagagg ctttttgctt cctcttgcaa aaccacactg ctcgactagt gattaaagtc
     2401 gactcgggga caccaaatat ggcgatctcg gccttttcgt ttcttggagc tgggacatgt
     2461 ttgccatcga tccatctacc accagaacgg ccgttagatc tgctgccacc gttgtttcca
     2521 ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa gctgccaccg
     2581 ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctcatgtt atttctggca
     2641 cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt gtcgttgatg
     2701 cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt tcttgttgtt
     2761 cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt cctccatctc
     2821 ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     2881 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     2941 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     3001 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     3061 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     3121 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     3181 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     3241 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     3301 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     3361 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     3421 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     3481 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3541 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3661 aaactcatca atgtatctta tcatgtctgg atctgacatg gtaagtaagc ttataacttc
     3721 gtatagcata cattatacga agttatcggc gcgccgcggc cgcgatcaat tcggtaccga
     3781 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcctcg agggtacccc
     3841 aattccgccc cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     3901 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3961 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4021 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4081 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4141 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4201 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4261 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4321 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     4381 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     4441 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4501 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4561 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4621 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4681 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4741 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4801 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4861 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4921 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4981 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5041 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5101 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatcc gggatccgga
     5161 gagctcccaa cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
     5221 tcgaggttgg atgcagcccg ggggatccac tagttctaga gcggccgatc agcctcgact
     5281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5521 accagctggg gctcgagatc cactagttct agcctcgagg ctagagcggc cgccgcggat
     5581 atcgaattcg ctagaagatg ggcgggagtc ttctgggcag gcttaaaggc taacctggtg
     5641 tgtgggcgtt gtcctgcagg ggaattgaac aggtgtaaaa ttggagggac aagacttccc
     5701 acagattttc ggttttgtcg ggaagttttt taataggggc aaataggaaa atggaggata
     5761 ggagtcatct ggggtttatg cagcaaaact acaggtatat tgcttgtatc cgcctcggag
     5821 atttccatga ggagataaag acatgtcacc cgagtttata ctctcctgct tagatcctac
     5881 tacagtatga aatacagtgt gcgaggtaga ctatgtaagc agatttaatc attttaaaga
     5941 gcccagtact tcatatccat ttctcccgct ccttctgcag ccttatcaaa aggtatttag
     6001 aacactcatt ttagccccat tttcatttat tatactggct tatccaaccc ctagacagag
     6061 cattggcatt ttccctttcc tgatcttaga agtctgatga ctcatgaaac cagacagatt
     6121 agttacatac accacaaatc gaggctgtag ctggggcctc aacactgcag ttcttttata
     6181 actccttagt acactttttg ttgatccttt gccttgatcc ttaattttca gtgtctatca
     6241 cctctcccgt caggtggtgt tccacatttg ggcctattct cagtccaggg agttttacaa
     6301 caatagatgt attgagaatc caacctaaag cttaactttc cactcccatg aatgcctctc
     6361 tcctttttct ccattataac tgagctatac cattaatggt ttcaggtgga tgtctcctcc
     6421 cccaatatac ctgatgtatc tacatattgc caggctgata ttttaagaca taaaggtata
     6481 tttcattatt gagccacatg gtattgatta ctgctactaa aattttgtca ttgtacacat
     6541 ctgtaaaagg tggttccttt tggaatgcaa agttcaggtg tttgttgtct ttcctgacct
     6601 aaggtcttgt gagcttgtat tttttctatt taagcagtgc tttctcttgg actggcttga
     6661 ctcatggcat tctacacgtt attgctggtc taaatgtgat tttgccaagc ttcttcagga
     6721 cctataattt tgcttgactt gtagccaaac acaagtaaaa tgattaagca acaaatgtat
     6781 ttgtgaagct tggtttttag gttgttgtgt tgtgtgtgct tgtgctctat aataatacta
     6841 tccaggggct ggagaggtgg ctcggagttc aagagcacag actgctcttc cagaagtcct
     6901 gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatgcc
     6961 ctcttctggt gtgtctgaag accacaagtg tattcacatt aaataaataa tcctccttct
     7021 tcttcttttt ttttttttaa agagaatctg tctccagtag aattactgaa gtaatgaaat
     7081 actttgtgtt tgttccaata tggagccaat aatcaaatac tcttagcact ggaaatgtac
     7141 caaggaacta ttttatttaa gtgactgtgg acagaggagc cataactgca gacttgtggg
     7201 atacagaaga ccaatgcaga cttaatgtct tttctcttac actaagcaat aaagaaataa
     7261 aaattgaact tctagtatcc tatttgttaa actgctagct ttactaactt ttgtgcttca
     7321 tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc aagtaatgat
     7381 aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg tgcttgcctt
     7441 tatgccttaa tcccagcact ctggaggcag agacaggcag atctctgagt ttgagcccag
     7501 cctggtctac acatcaagtt ctatctagga tagccaggaa tacacacaga aaccctgttg
     7561 gggagggggg ctctgagatt tcataaaatt ataattgaag cattccctaa tgagccacta
     7621 tggatgtggc taaatccgtc tacctttctg atgagatttg ggtattattt tttctgtctc
     7681 tgctgttggt tgggtctttt gacactgtgg gctttcttaa agcctccttc cctgccatgt
     7741 ggtctcttgt ttgctactaa cttcccatgg cttaaatggc atggcttttt gccttctaag
     7801 ggcagctgct gagtttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact
     7861 aaaattgtcc tttaattttt ttttaaaaaa tgggttatat aataaacctc ataaaatagt
     7921 tatgaggagt gaggtggact aatattaatg agtccctccc ctataaaaga gctattaagg
     7981 ctttttgtct tatactaact ttttttttaa atgtggtatc tttagaacca agggtcttag
     8041 agttttagta tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga
     8101 gaatccaaat tcttcacagc caaagtcaaa ttaagaattt ctgactttaa tgttatttgc
     8161 tactgtgaat ataaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct
     8221 gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg
     8281 ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta
     8341 agggagcttg ggtgatagtt ggtaaatgtg tttaagtgat gaaaacttga attattatca
     8401 ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctaa gggatcccta
     8461 ggacttgctg agcacacaag agtagtactt ggcaggctcc tggtgagagc atatttcaaa
     8521 aaacaaggca gacaaccaag aaactacagt aaggttacct gtctttaacc atctgcatat
     8581 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
     8641 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
     8701 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
     8761 tttcgaggtc tcttggttat actcagaagc acttctttgg gtttccctgc actatcctga
     8821 tcatgtgcta ggcctcctta ggctgattgt tgttcaaata acttaagttt cctgtcaggt
     8881 gatgtcatat gatttcatat atcaaggcaa aacatgttat atatgttaaa catttgactt
     8941 aatgtgaaag ttaggtcttt gtgggttttg attttaattt caaaacctga gctaaataag
     9001 tcattttaca tgtcttacat ttggtgaatt gtatattgtg gtttgcaggc aagactctct
     9061 gacctagtaa ccctcctata gagcactttg ctgggtcaca agtctaggag tcaagcattt
     9121 caccttgaag ttgagacgtt ttgttagtgt atactagtta tatgttggag gacatgttta
     9181 tccagaagat attcaggact atttttgact gggctaagga attgattctg attagcactg
     9241 ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat ctcaaataat
     9301 gctggccttt tttaaaagcc cttgttcttt atcaccctgt tttctacata atttttgttc
     9361 aaagaaatac ttgtttggat ctccttttga caacaatagc atgttttcaa gccatatttt
     9421 ttttcctttt tttttttttt tttggttttt cgagacaggg tttctctgta tagccctggc
     9481 tgtcctggaa ctcactttgt agaccaggct ggcctcgaac tcagaaatcc gcctgcctct
     9541 gcctcctgag tgccgggatt aaaggcgtgc accaccacgc ctggctaagt tggatatttt
     9601 gtatataact ataaccaata ctaactccac tgggtggatt tttaattcag tcagtagtct
     9661 taagtggtct ttattggccc ttattaaaat ctactgttca ctctaacaga ggctgttgga
     9721 ctagtggact aagcaacttc ctacggatat actagcagat aagggtcagg gatagaaact
     9781 agtctagcgt tttgtatacc taccagctta tactaccttg ttctgataga aatatttagg
     9841 acatctagct tatcgatccg tcgacggtat cgataagctt gatatcgaat tctaccgggt
     9901 aggggaggcg cttttccaag gcagtctgag catgcgctta gcagccccgc tggcacttgg
     9961 cgctacacaa gtggccttgg cctcgcacac attccacatc caccggtagg cgccaaccgg
    10021 ctccgttctt tggtggcccc ttcgcgccac cttctctcct cccctagtca ggaagttccc
    10081 ccccgccccg cagctcgcgt cgtaggacgt gacaaatgga agtagcacgt ctcactagtc
    10141 tcgtcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    10201 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    10261 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
    10321 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    10381 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttattct tctaatcttt
    10441 tgtatggaaa acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa
    10501 aaaggtatac aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg
    10561 ttttatagta ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg
    10621 ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc
    10681 gcactaaaag tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa
    10741 ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg
    10801 cgtgtagtgc tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac
    10861 tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa
    10921 cgtggccaag atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg
    10981 cgatctcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    11041 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    11101 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    11161 gtggaatgca gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
    11221 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    11281 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    11341 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    11401 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacctc
    11461 gagggggggc ccggtaccgc ggccgcgatc gcctgcaggt acgtagtacc agcttttgtt
    11521 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    11581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    11641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    11701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    11761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    11881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    12001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    12181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    12421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    12481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    12541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    12601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    12661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    12841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    12901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    12961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    13021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    13441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    13501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    13561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    13621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    13681 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
    13741 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
    13801 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
    13861 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
    13921 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
    13981 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
    14041 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
    14101 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
    14161 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    14221 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    14281 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
    14341 cgactcacta tagggcgaat tggagct
//
