LOCUS       sequence-70572-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             115..141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
       61 ttttcgcaac gggtttgccg ccagaacaca ggatctgccg ccaccatggg cgtatacccc
      121 tacgacgtgc ccgactacgc ccccgggccc cctaagaaaa agaggaaggt gaagaacatc
      181 aagaagaacc aggtcatgaa cctgggcccc aacagcaagc tgctgaagga gtacaagagc
      241 cagctgatcg agctgaacat cgagcagttc gaggccggca tcggcctgat cctgggcgac
      301 gcctacatca ggagcaggga cgagggcaag acctactgca tgcagttcga gtggaagaac
      361 aaggcctaca tggaccacgt gtgcctgctg tacgaccagt gggtgctgag ccccccccac
      421 aagaaggaga gggtgaacca cctgggcaac ctggtcatca cctggggcgc ccagaccttc
      481 aagcaccagg ccttcaacaa gctggccaac ctgttcatcg tgaacaacaa gaagaccatc
      541 cccaacaacc tggtggagaa ctacctgacc cccatgagcc tggcctactg gttcatggac
      601 gacggcggca agtgggacta caacaagaac agcaccaaca agagcatcgt gctgaacacc
      661 cagagcttca ccttcgagga ggtggagtac ctggtgaagg gcctgaggaa caagttccag
      721 ctgaactgct acgtgaagat caacaagaac aagcccatca tctacatcga cagcatgagc
      781 tacctgatct tctacaacct gatcaagccc tacctgatcc cccagatgat gtacaagctg
      841 cccaacacca tcagcagcga gaccttcctg aagggcggcg gcggatccgg tgagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatccgg gccccatgag cgagctgatt
      961 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaa
//
