LOCUS       sequence-70575-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses iRFP under the CaM Kinase IIa 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC364 - pAAV CaMKIIa iRFP
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..1008
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 gggcaggcga gtggccccta gttctggggg cagctctaga gcggtaccgg atccaccatg
       61 gctgaaggat ccgtcgccag gcagcctgac ctcttgacct gcgacgatga gccgatccat
      121 atccccggtg ccatccaacc gcatggactg ctgctcgccc tcgccgccga catgacgatc
      181 gttgccggca gcgacaacct tcccgaactc accggactgg cgatcggcgc cctgatcggc
      241 cgctctgcgg ccgatgtctt cgactcggag acgcacaacc gtctgacgat cgccttggcc
      301 gagcccgggg cggccgtcgg agcaccgatc actgtcggct tcacgatgcg aaaggacgca
      361 ggcttcatcg gctcctggca tcgccatgat cagctcatct tcctcgagct cgagcctccc
      421 cagcgggacg tcgccgagcc gcaggcgttc ttccgccgca ccaacagcgc catccgccgc
      481 ctgcaggccg ccgaaacctt ggaaagcgcc tgcgccgccg cggcgcaaga ggtgcggaag
      541 attaccggct tcgatcgggt gatgatctat cgcttcgcct ccgacttcag cggcgaagtg
      601 atcgcagagg atcggtgcgc cgaggtcgag tcaaaactag gcctgcacta tcctgcctca
      661 accgtgccgg cgcaggcccg tcggctctat accatcaacc cggtacggat cattcccgat
      721 atcaattatc ggccggtgcc ggtcacccca gacctcaatc cggtcaccgg gcggccgatt
      781 gatcttagct tcgccatcct gcgcagcgtc tcgcccgtcc atctggaatt catgcgcaac
      841 ataggcatgc acggcacgat gtcgatctcg attttgcgcg gcgagcgact gtggggattg
      901 atcgtttgcc atcaccgaac gccgtactac gtcgatctcg atggccgcca agcctgcgag
      961 ctagtcgccc aggttctggc ctggcagatc ggcgtgatgg aagagtgagt cgacgaattc
     1021 gat
//
