LOCUS       sequence-70576-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, 
            double floxed inverted open reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC374 - pAAV EF1a DIO iRFP
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(130..163)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttcaggtgt cgtgaggtac cggatcctct agagtcgact ccggaataac ttcgtatagg
       61 atactttata cgaagttatg cagaatggta gctggattgt agctgctatt agcaatatga
      121 aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc cggtaccgtc
      181 gactcactct tccatcacgc cgatctgcca ggccagaacc tgggcgacta gctcgcaggc
      241 ttggcggcca tcgagatcga cgtagtacgg cgttcggtga tggcaaacga tcaatcccca
      301 cagtcgctcg ccgcgcaaaa tcgagatcga catcgtgccg tgcatgccta tgttgcgcat
      361 gaattccaga tggacgggcg agacgctgcg caggatggcg aagctaagat caatcggccg
      421 cccggtgacc ggattgaggt ctggggtgac cggcaccggc cgataattga tatcgggaat
      481 gatccgtacc gggttgatgg tatagagccg acgggcctgc gccggcacgg ttgaggcagg
      541 atagtgcagg cctagttttg actcgacctc ggcgcaccga tcctctgcga tcacttcgcc
      601 gctgaagtcg gaggcgaagc gatagatcat cacccgatcg aagccggtaa tcttccgcac
      661 ctcttgcgcc gcggcggcgc aggcgctttc caaggtttcg gcggcctgca ggcggcggat
      721 ggcgctgttg gtgcggcgga agaacgcctg cggctcggcg acgtcccgct ggggaggctc
      781 gagctcgagg aagatgagct gatcatggcg atgccaggag ccgatgaagc ctgcgtcctt
      841 tcgcatcgtg aagccgacag tgatcggtgc tccgacggcc gccccgggct cggccaaggc
      901 gatcgtcaga cggttgtgcg tctccgagtc gaagacatcg gccgcagagc ggccgatcag
      961 ggcgccgatc gccagtccgg tgagttcggg aaggttgtcg ctgccggcaa cga
//
