LOCUS       sequence-7058-ex       11627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB
FEATURES             Location/Qualifiers
     source          1..11627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2184..2683
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2219..2242)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2616..2635
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2734..2743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2740..2742,2743..2745,2746..3456)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2740..2742,2743..2745,2746..3456)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2785..2806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3046..3065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3393..3414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3532..4099
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3550..4101
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3694..3711)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3921..3940
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4104..4724
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4177..4196)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4725..4745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4848..5042
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5180..5196)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5181..5197)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5211..5799
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5264..5284)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5682..5693)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5937..5955,5956..5978,5979..5997,5998..6020,6021..6039,
                     6040..6062,6063..6081,6082..6104,6105..6123,6124..6146,
                     6147..6165,6166..6188,6189..6207)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6242..6422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6447..6465)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6448..6465)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6755..6774)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6988..7007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7107..7129
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7167..7185)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7253..7357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7358..7426,7427..8218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7576..7595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8389..8977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8878..8897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9131..9148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9218..9238)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9223..9552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9403..9538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10686..10751
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10881..10901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11326..11460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11363..11382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11417..11436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     2161 agcctcgacg atggtcgagt accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2221 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     2281 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     2341 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     2401 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     2461 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     2521 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     2581 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     2641 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta gcgggatcca
     2701 ggcctaagct tacgcgtcct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
     2761 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2821 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2881 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2941 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3001 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3061 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3121 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3181 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3241 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3301 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3361 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3421 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgatagcta
     3481 gtagctagct agctagtcga gctcaacttc gaattcccgg gctgcaggaa ttccgccccc
     3541 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3601 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3661 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3721 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     3781 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     3841 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     3901 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     3961 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     4021 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     4081 ttgaaaaaca cgatgataat accatggcta gattagataa aagtaaagtg attaacagcg
     4141 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     4201 agcttggtgt agagcagcct acactgtatt ggcatgtaaa aaataagcgg gctttgctcg
     4261 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttaaaagggg
     4321 aaagctggca agatttttta cgcaataacg ctaaaagttt tagatgtgct ttactaagtc
     4381 atcgcaatgg agcaaaagta cattcagata cacggcctac agaaaaacag tatgaaactc
     4441 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaac gcgttatatg
     4501 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     4561 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     4621 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     4681 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcgccaaaa aagaagagaa
     4741 aggtcgacgg cggtggtgct ttgtctcctc agcactctgc tgtcactcaa ggaagtatca
     4801 tcaagaacaa ggagggcatg gatgctaagt cactaactgc ctggtcccgg acactggtga
     4861 ccttcaagga tgtatttgtg gacttcacca gggaggagtg gaagctgctg gacactgctc
     4921 agcagatcgt gtacagaaat gtgatgctgg agaactataa gaacctggtt tccttgggtt
     4981 atcagcttac taagccagat gtgatcctcc ggttggagaa gggagaagag ccctggctgg
     5041 tggagagaga aattcaccaa gagacccatc ctgattcaga gactgcattt gaaatcaaat
     5101 catcagttta aggatctcga ctagctagta gctagctagc tagtcgagct caacttcgaa
     5161 ttcgatatca agcttatcgc gataccgtcg acctcgacta gtcatatgat aatcaacctc
     5221 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5281 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5341 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5401 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5461 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5521 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5581 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5641 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5701 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5761 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtacgta tggccaggta
     5821 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5881 gggggactgg aagggctaat tcactcccaa cgaagacaag atctcgagtt taccactccc
     5941 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     6001 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6061 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6181 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg gatctgcttt ttgcttgtac
     6241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6301 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6361 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6421 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     6481 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     6541 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     6601 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     6661 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     6721 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     6781 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     6841 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     6901 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     6961 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7081 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7141 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7201 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8521 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8761 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9181 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     9241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9301 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9361 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9541 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     9601 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     9661 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     9721 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     9781 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     9841 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     9901 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     9961 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
    10021 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
    10081 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
    10141 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
    10201 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
    10261 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
    10321 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
    10381 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
    10441 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
    10501 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
    10561 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
    10621 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
    10681 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
    10741 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
    10801 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
    10861 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
    10921 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
    10981 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
    11041 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
    11101 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
    11161 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
    11221 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    11281 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    11341 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11401 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11461 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11521 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    11581 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtag
//
