LOCUS       sequence-70589-e        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of fluorescent Drosophila LIS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Jodoin JN, Shboul M, Sitaram P, Zein-Sabatto H, Reversade B, Lee E, 
            Lee LA
  TITLE     Human Asunder promotes dynein recruitment and centrosomal tethering 
            to the nucleus at mitotic entry.
  JOURNAL   Mol Biol Cell. 2012 Dec;23(24):4713-24. doi: 
            10.1091/mbc.E12-07-0558. Epub 2012 Oct 24.
  PUBMED    23097494
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-07-0558"; journalName: "Mol Biol Cell"; date: 
            "2012-12"; volume: "23"; issue: "24"; pages: "4713-24"
COMMENT     Sequence Label: CS2-CHY-dLIS1
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..725
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKESMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(163..181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     431..450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttctttttgc aggatccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
       61 agtccatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      121 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      181 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      241 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      301 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      361 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      421 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      481 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      541 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      601 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      661 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      721 acaagggccg gccaatgaaa atggtgttgt cgcagcggca gcgcgaggag cttaaccaag
      781 cgattgccga ttatctgggc tcaaatggct acgccgactc attggagacc ttccgcaagg
      841 aggccgatct cagcacagaa gtggaaaaga aatttggcgg actgttggaa aagaagtgga
      901 cgtccgtgat tcggctgcag aagaaagtga tggaactaga ggctaagctc actgaagctg
      961 agaaggaggt catcgagggg gcgcccacaa agaacaaacg gacgcctggc gagtggatac
     1021 ctcggccgcc agagaagttc tccctgaccg gtcatcgtgc cagcataacg agggtaattt
     1081 tccaccccat ctttgctctt atggtttccg catccg
//
