LOCUS       sequence-7059-ex       12859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (CAG 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 12859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVCT-rtTR-KRAB-2SM2
FEATURES             Location/Qualifiers
     source          1..12859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             6..639
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    686..811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2061..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2442..2717
                     /label=chicken beta-actin promoter
     intron          2718..3728
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3652..3674
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3736..3755
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    3814..3932
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4009..4018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4015..4017,4018..4020,4021..4731)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4015..4017,4018..4020,4021..4731)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4060..4081)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4321..4340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4668..4689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4792..5359
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4810..5361
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4954..4971)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5181..5200
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5985..6005
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6108..6302
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    6443..7031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6496..6516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6914..6925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(7169..7187,7188..7210,7211..7229,7230..7252,7253..7271,
                     7272..7294,7295..7313,7314..7336,7337..7355,7356..7378,
                     7379..7397,7398..7420,7421..7439)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             7478..7658
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7683..7701)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7684..7701)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7991..8010)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8224..8243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8343..8365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8403..8421)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8489..8593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8594..8662,8663..9454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8812..8831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9625..10213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10114..10133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10367..10384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10454..10474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10459..10788
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10639..10774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11922..11987
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12117..12137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12562..12696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12599..12618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12653..12672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
       61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
      121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
      181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
      241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
      301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
      361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
      421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg
     2581 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     2641 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     2701 gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg
     2761 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     2821 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc
     2881 tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc
     2941 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg
     3001 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg
     3061 gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc
     3121 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa
     3181 cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     3241 ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     3301 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     3361 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     3421 tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc
     3481 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     3541 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     3601 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     3661 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     3721 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     3781 taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     3841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa tttaaagaat
     3901 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt
     3961 aaactacggg atccaggcct aagcttacgc gtcctagcgc taccggtcgc caccatggtg
     4021 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4081 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4201 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4321 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4441 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4501 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4621 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     4741 agatctcgac tagctagtag ctagctagct agtcgagctc aagcttcgaa ttccgccccc
     4801 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4861 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4921 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4981 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5041 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5101 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5161 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5221 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5281 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5341 ttgaaaaaca cgatgataat accatggcta gactggacaa gagcaaagtc ataaacggcg
     5401 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     5461 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     5521 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     5581 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
     5641 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     5701 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     5761 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     5821 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     5881 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     5941 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcgccaaaa aagaagagaa
     6001 aggtcgacgg cggtggtgct ttgtctcctc agcactctgc tgtcactcaa ggaagtatca
     6061 tcaagaacaa ggagggcatg gatgctaagt cactaactgc ctggtcccgg acactggtga
     6121 ccttcaagga tgtatttgtg gacttcacca gggaggagtg gaagctgctg gacactgctc
     6181 agcagatcgt gtacagaaat gtgatgctgg agaactataa gaacctggtt tccttgggtt
     6241 atcagcttac taagccagat gtgatcctcc ggttggagaa gggagaagag ccctggctgg
     6301 tggagagaga aattcaccaa gagacccatc ctgattcaga gactgcattt gaaatcaaat
     6361 catcagttta aggatctcga ctagctagta gctagctagc tagtcgagct caacttcgaa
     6421 ttcccgggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6481 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6541 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6601 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6661 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6721 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6781 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6841 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6901 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6961 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7021 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag
     7081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7141 aacgaagaca agatctcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     7201 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     7261 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     7321 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     7381 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     7441 aagtgaaagt cggatcgatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7501 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7561 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7621 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     7681 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     7741 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     7801 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     7861 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     7921 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     7981 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     8041 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     8101 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     8161 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     8221 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     8281 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8341 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     8401 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     8461 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8761 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9001 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9301 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9421 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9481 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9541 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     9781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9901 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9961 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
    10021 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10081 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10141 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10201 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10261 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10321 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10381 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    10441 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    10501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    10561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    10621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    10741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
    10801 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
    10861 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
    10921 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
    10981 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
    11041 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
    11101 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
    11161 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
    11221 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
    11281 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
    11341 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
    11401 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    11461 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    11521 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    11581 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    11641 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    11701 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    11761 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    11821 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    11881 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    11941 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    12001 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    12061 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    12121 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    12181 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    12241 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    12301 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    12361 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    12421 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    12481 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    12541 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    12601 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    12661 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    12721 aatcatccca aacttcccac cccataccct attaccactg ccaattacct agtggtttca
    12781 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12841 aatatgtact acaaactta
//
