LOCUS       sequence-70606-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA under the control of GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pCH043
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(81..521)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(87..110)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    405..521
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 tccgacccca ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctagg
       61 catggatcct atagtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt
      121 tgttgatact tttatgacat ttgaataaga agtaatacaa actgaaaatg ttgaaagtat
      181 tagttaaagt ggttatgcag cttttccatt tatatatctg ttaatagatc aaaaatcatc
      241 gcttcgctga ttaattaccc cagaaataag gctaaaaaac taatcgcatt atcatcctat
      301 ggttgttaat ttgattcgtt aatttgaagg tttgtggggc caggttactg ccaatttttc
      361 ctcttcataa ccataaaagc tagtattgta gaatctttat tgttcggagc agtgcggcgc
      421 gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc
      481 ttccgtcgga gggctgtcgc ccgctcggcg gcttctaatc ctacttcaat atgcatgcgg
      541 accactctta cataactaga atagcattaa gaatcagatt tacagataat gatgtcatta
      601 ttaaatatat atatatatat attgtcactc cgttcaagtc gacaaccaat aaaaaattta
      661 aaagaaacca ggcagttaat agaaaaaata tgatatgaat gaatattcca ctttcttttt
      721 acagtctttg tagataatga atctgaccct cgagctaaac tttggaatgc aatgtagatg
      781 aatccgaaga gtagttattg tacctatttt gggcacatgg aacattatca atttcatctt
      841 tcaagctttt cctatccctt aaaaatatta accataaaac cacactgtct aaaaggagga
      901 atgcaaagct aaaccaagcc attactgctg ccttcttgcc cttcttgaca gtgattatat
      961 tcatacttga tgatggaacc aatctgtaac ttgcatttat ccctacatga gtccacatcg
     1021 caccaatacc ccatgtcaaa actatagttg aacttagcaa cagtagaaat ttattaaccg
     1081 ctagtttgaa tggtaactta gggacagtt
//
