LOCUS       sequence-70622-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA positive feedback loop with three tet operators in the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pR184
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(85..108)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    466..484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    486..504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    507..525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgaccccatt aagcagctct aatgcgctgt taatcacttt acttttatct aatctaggca
       61 tggatcttat agttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg
      121 ttgatacttt tatggcattt gaataagaag taatacaaac tgaaaatgtt gaaagtatta
      181 gttaaagtgg ttatgcagct tttccattta tatatctgtt aatagatcaa aaatcatcgc
      241 ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat catcctatgg
      301 ttgttaattt gattcgttaa tttgaaggtt tgtggggcca ggttactgcc aatttttcct
      361 cttcataacc ataaaagcta gtattgtaga atctttattg ttgccagcag tgcggcggca
      421 ggcacatctg ctttcaggaa cgcgaccggt gaagacgagg acgcatctct atcactgata
      481 gggattctct atcactgata gggacttctc tatcactgat agggatactt caatatccta
      541 ggggaccact cttacataac tagaatagca ttaagaatca gatttacaga taatgatgtc
      601 attattaaat atatatatat atatattgtc actccgttca agtcgacaac caataaaaaa
      661 tttaaaagaa accaggcagt taatagaaaa aatatgatat gaatgaatat tccactttct
      721 ttttacagtc tttgtagata atgaatctga ccctcgagct aaactttgga atgcaatgta
      781 gatgaatccg aagagtagtt attgtaccta ttttgggcac atggaacatt atcaatttca
      841 tctttcaagc ttttcctatc ccttaaaaat attaaccata aaaccacact gtctaaaagg
      901 aggaatgcaa agctaaacca agccattact gctgccttct tgcccttctt gacagtgatt
      961 atattcatac ttgatgatgg aaccaatctg taacttgcat ttatccctac atgagtccac
     1021 atcgcaccaa taccccatgt caaaactata gttgaactta gcaacagta
//
