LOCUS       sequence-70623-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA positive feedback loop with three tet operators in the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pR184
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(48..434,435..1055))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MPRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     963..982
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gatgcggccc tatttgtaca gaattcttat tacgatcctc gcgcccccta cccaccgtac
       61 tcgtcaattc caagggcatc ggtaaacatc tgctcaaact cgaagtcggc catatccaga
      121 gcgccgtagg gggcggagtc gtggggggta aatcccggac ccggggaatc cccgtccccc
      181 aacatgtcca gatcgaaatc gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg
      241 tctaagtgga gctcgtcccc caggctgaca tcggtcgggg gggccgtcga cagtctgcgc
      301 gtgtgtcccg cggggagaaa ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg
      361 tcgtcgtccg ggagatcgag caggccctcg atggtagacc cgtaattgtt tttcgtacgc
      421 gcgcggctgt acgcggaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
      481 aattcaaggc cgaataagaa ggctggctct gcaccttggc gatcaaataa ttcgatagct
      541 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
      601 tgatgctctt gttcttccaa tacgcaacct aaagtaaagt gccccacagc gctgagtgca
      661 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
      721 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
      781 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
      841 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggg taaggcgtcg
      901 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
      961 tgggcgagtt tacgggttgt taaaccttcg attccgaccc cattaagcag ctctaatgcg
     1021 ctgttaatca ctttactttt atctaatcta ggcatggatc ttatagttt
//
