LOCUS       sequence-70624-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA positive feedback loop with three tet operators in the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pR184
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             216..1019
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ttcattcatc attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg
       61 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
      121 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
      181 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
      241 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
      301 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
      361 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
      421 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
      481 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
      541 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
      601 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
      661 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
      721 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
      781 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
      841 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
      901 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
      961 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
     1021 aaactgtatt ataagtaaat gcatgtatac taaactc
//
