LOCUS       sequence-70625-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA positive feedback loop with three tet operators in the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pR184
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..40)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        108..212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(213..281,282..1073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(431..450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
       61 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1081 a
//
