LOCUS       sequence-70626-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetO-GFP reporter casette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pCH001
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      99..286
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(154..178)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc
       61 gggccccccc tcgagtaatt cgcgccactt ctaaataagc gaatttctta tgatttatga
      121 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
      181 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
      241 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
      301 ataacagggt aatatagatc aattcctcga tccctatcag tgatagagag tcgacaaagt
      361 cgagtttctc gatcgagacc actgcatgca tgtgctctgt atgtatataa aactcttgtt
      421 ttcttctttt ctctaaatat tctttcctta tacattaggt cctttgtagc ataaattact
      481 atacttctat agacacgcaa acacaaatac acacactaaa ttaccggatc aattcggggg
      541 atccaaaatg tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
      601 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
      661 ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc
      721 aaccttagtc actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat
      781 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
      841 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
      901 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
      961 tcacaaattg gaatacaact ataactctca caatgtttac atcatggctg acaaacaaa
//
