LOCUS       sequence-70627-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetO-GFP reporter casette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pCH001
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..744)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    943..961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gatgcggccc tatttgtaca gaattcttta tttgtacaat tcatccatac catgggtaat
       61 accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt
      121 ggataaggca gattgagtgg ataagtaatg gttgtctggt aacaagactg gaccatcacc
      181 aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg
      241 tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt
      301 gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc
      361 aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct
      421 ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg
      481 catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctcg caaaacattg
      541 aacaccataa ccgaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt
      601 acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac
      661 agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga caacaccagt
      721 gaataattct tcacctttag acattttgga tcccccgaat tgatccggta atttagtgtg
      781 tgtatttgtg tttgcgtgtc tatagaagta tagtaattta tgctacaaag gacctaatgt
      841 ataaggaaag aatatttaga gaaaagaaga aaacaagagt tttatataca tacagagcac
      901 atgcatgcag tggtctcgat cgagaaactc gactttgtcg actctctatc actgataggg
      961 atcgaggaat tgatctatat taccctgtta tccctagcgg atctgccggt agaggtgtgg
     1021 tcaataagag cgacctcata ctatacctga gaaagcaacc tgacctacag gaaagagtta
     1081 ctcaagaata
//
