LOCUS       sequence-70636-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BglBrick plasmid 
            (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV 
            pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pJBEI-6410
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(61..66,67..84,85..91)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    99..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(850..861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgtttagg caccccaggc
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      121 gaattcaaaa gatcttagga ggaatataaa atgaaaaatt gtgtcatcgt cagtgcggta
      181 cgtactgcta tcggtagttt taacggttca ctcgcttcca ccagcgccat cgacctgggg
      241 gcgacagtaa ttaaagccgc cattgaacgt gcaaaaatcg attcacaaca cgttgatgaa
      301 gtgattatgg gtaacgtgtt acaagccggg ctggggcaaa atccggcgcg tcaggcactg
      361 ttaaaaagcg ggctggcaga aacggtgtgc ggattcacgg tcaataaagt atgtggttcg
      421 ggtcttaaaa gtgtggcgct tgccgcccag gccattcagg caggtcaggc gcagagcatt
      481 gtggcggggg gtatggaaaa tatgagttta gccccctact tactcgatgc aaaagcacgc
      541 tctggttatc gtcttggaga cggacaggtt tatgacgtaa tcctgcgcga tggcctgatg
      601 tgcgccaccc atggttatca tatggggatt accgccgaaa acgtggctaa agagtacgga
      661 attacccgtg aaatgcagga tgaactggcg ctacattcac agcgtaaagc ggcagccgca
      721 attgagtccg gtgcttttac agccgaaatc gtcccggtaa atgttgtcac tcgaaagaaa
      781 accttcgtct tcagtcaaga cgagttcccg aaagcgaact caacggctga agcgttaggt
      841 gcattgcgcc cggccttcga taaagcagga acagtcaccg ctgggaacgc gtctggtatt
      901 aacgacggtg ctgccgc
//
