LOCUS       sequence-70637-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid separately encodes a human codon-optimized SpCas9, a 
            tracrRNA and customizable crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: 
            pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..155
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     repeat_region   216..251
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   268..303
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
       61 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      121 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      181 gctttatata tcttgtggaa aggacgaaac accgggtttt agagctatgc tgttttgaat
      241 ggtcccaaaa cgggtcttcg agaagacgtt ttagagctat gctgttttga atggtcccaa
      301 aactttttct agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt acccgttaca
      361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      541 gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact
      601 tggcagtaca tctacgtatt agtcatc
//
