LOCUS       sequence-70638-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BglBrick plasmid 
            (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV 
            pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pJBEI-6410
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(162..189)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(205..276)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
       61 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
      121 agtcagtgag cgaggaagcg gaatatatcc ctaggtataa acgcagaaag gcccacccga
      181 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
      241 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggagatcc ttactcgagt
      301 ttggatcctt aggcgaaagg tgcaaacagg gtacgcgtca tctgctgatg aataatagga
      361 tgctgggtcc catgaccatc accgttatga tacatcagct gggccatacg acccagatca
      421 acggcgcaac caataaaatc cttaccaaac ggactatcct tggaaacacg ttcagcgttc
      481 atcttcttcc acacttcggc gatcagccac ttcacatgtt tgcgagcttc agcttcactt
      541 gcgttataat cggacatgta gcactgtaag gatttcggaa catccccacg agacacctct
      601 tccacagacg tacccagatc gtccgccagc cgcaggacga aagatgacca acgaaccagg
      661 tcatggtatt tatataagga atcgaccgtc tccttggtaa atgaatccgt gacgcgaaag
      721 aagatatgcg tcagcatgca tggcccactg atactctgcc atgagttctc cagatattct
      781 tctaaactcg gtttgtgccc accgtagaac cagcgtgcct caaccatata tttatcggcc
      841 agatccaccc aactctgacg cagataaggg ataacattcc
//
