LOCUS       sequence-70640-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BglBrick plasmid 
            (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV 
            pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pJBEI-6409
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(164..191)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(207..278)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
       61 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
      121 cgagtcagtg agcgaggaag cggaatatat ccctaggtat aaacgcagaa aggcccaccc
      181 gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggagat ccttactcga
      301 gtttggatcc ttaggcgaaa ggtgcaaaca gggtacgcgt catctgctga tgaataatag
      361 gatgctgggt cccatgacca tcaccgttat gatacatcag ctgggccata cgacccagat
      421 caacggcgca accaataaaa tccttaccaa acggactatc cttggaaaca cgttcagcgt
      481 tcatcttctt ccacacttcg gcgatcagcc acttcacatg tttgcgagct tcagcttcac
      541 ttgcgttata atcggacatg tagcactgta aggatttcgg aacatcccca cgagacacct
      601 cttccacaga cgtacccaga tcgtccgcca gccgcaggac gaaagatgac caacgaacca
      661 ggtcatggta tttatataag gaatcgaccg tctccttggt aaatgaatcc gtgacgcgaa
      721 agaagatatg cgtcagcatg catggcccac tgatactctg ccatgagttc tccagatatt
      781 cttctaaact cggtttgtgc ccaccgtaga accagcgtgc ctcaaccata tatttatcgg
      841 ccagatccac ccaactctga cgcagataag ggataacatt cacgccttt
//
