LOCUS       sequence-7065-ex         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     Salvesen Casp1, Casp2 plasmids
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET23b-Casp2-Delta Card
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             878..895
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctagaaat aattttgttt aactttaaga aggagatata catatgaagc cttgcactcc
       61 tgaattttat caaacacact tccagctggc atataggttg cagtctcggc ctcgtggcct
      121 agcactggtg ttgagcaatg tgcacttcac tggagagaaa gaactggaat ttcgctctgg
      181 aggggatgtg gaccacagta ctctagtcac cctcttcaag cttttgggct atgacgtcca
      241 tgttctatgt gaccagactg cacaggaaat gcaagagaaa ctgcagaatt ttgcacagtt
      301 acctgcacac cgagtcacgg actcctgcat cgtggcactc ctctcgcatg gtgtggaggg
      361 cgccatctat ggtgtggatg ggaaactgct ccagctccaa gaggtttttc agctctttga
      421 caacgccaac tgcccaagcc tacagaacaa accaaaaatg ttcttcatcc aggcctgccg
      481 tggagatgag actgatcgtg gggttgacca acaagatgga aagaaccacg caggatcccc
      541 tgggtgcgag gagagtgatg ccggtaaaga aaagttgccg aagatgagac tgcccacgcg
      601 ctcagacatg atatgcggct atgcctgcct caaagggact gccgccatgc ggaacaccaa
      661 acgaggttcc tggtacatcg aggctcttgc tcaagtgttt tctgagcggg cttgtgatat
      721 gcacgtggcc gacatgctgg ttaaggtgaa cgcacttatc aaggatcggg aaggttatgc
      781 tcctggcaca gaattccacc ggtgcaagga gatgtctgaa tactgcagca ctctgtgccg
      841 ccacctctac ctgttcccag gacaccctcc cctcgagcac caccaccacc accactgaga
      901 tccggctgct aacaaagccc gaaaggaagc tgagttggct gct
//
