LOCUS       sequence-70650-e        7828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRed plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: p8RwB
FEATURES             Location/Qualifiers
     source          1..7828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2066..2086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2071..2400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2251..2386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2448..2495
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     polyA_signal    2975..3096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2986..3005)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3040..3059
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3279..4457
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3510..4448
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4405..4425
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4548..4557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4554..5228
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4684..4702)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4734..4754)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4952..4971
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5146..5169
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5287..5875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5340..5360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5758..5769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5917..6489
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      6660..7248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7149..7168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7334..7381
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7373..7391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcatca agcttggtac cgagctcgga tctaaataaa cttataaatt gtgagagaaa
       61 ttaatgaatg tctaagttaa tgcagaaacg gagagacata ctatattcat gaactaaaag
      121 acttaatatt gtgaaggtat actttctttc cacataaatt tgtagtcaat atgttcaccc
      181 caaaaaagct gtttgttaac ttgccaacct cattctaaaa tgtatataga agcccaaaag
      241 acaataacaa aaatattctt gtagaacaaa atgggaaaga atgttccact aaatatcaag
      301 atttagagca aagcatgaga tgtgtgggga tagacagtga ggctgataaa atagagtaga
      361 gctcagaaac agacccattg atatatgtaa gtgacctatg aaaaaaatat ggcattttac
      421 aatgggaaaa tgatgatctt tttctttttt agaaaaacag ggaaatatat ttatatgtaa
      481 aaaataaaag ggaacccata tgtcatacca tacacacaaa aaaattccag tgaattataa
      541 gtctaaatgg agaaggcaaa actttaaatc ttttagaaaa taatatagaa gcatgccatc
      601 atgacttcag tgtagagaaa aatttcttat gactcaaagt cctaaccaca aagaaaagat
      661 tgttaattag attgcatgaa tattaagact tatttttaaa attaaaaaac cattaagaaa
      721 agtcaggcca tagaatgaca gaaaatattt gcaacacccc agtaaagaga attgtaatat
      781 gcagattata aaaagaagtc ttacaaatca gtaaaaaata aaactagaca aaaatttgaa
      841 cagatgaaag agaaactcta aataatcatt acacatgaga aactcaatct cagaaatcag
      901 agaactatca ttgcatatac actaaattag agaaatatta aaaggctaag taacatctgt
      961 ggcaatattg atggtatata accttgatat gatgtgatga gaacagtact ttaccccatg
     1021 ggcttcctcc ccaaaccctt accccagtat aaatcatgac aaatatactt taaaaaccat
     1081 taccctatat ctaaccagta ctcctcaaaa ctgtcaaggt catcaaaaat aagaaaagtc
     1141 tgaggaactg tcaaaactaa gaggaaccca aggagacatg agaattatat gtaatgtggc
     1201 attctgaatg agatcccaga acagaaaaag aacagtagct aaaaaactaa tgaaatataa
     1261 ataaagtttg aactttagtt ttttttaaaa aagagtagca ttaacacggc aaagccattt
     1321 tcatattttt cttgaacatt aagtacaagt ctataattaa aaatttttta aatgtagtct
     1381 ggaacattgc cagaaacaga agtacagcag ctatctgtgc tgtcgcctaa ctatccatag
     1441 ctgattggtc taaaatgaga tacatcaacg ctcctccatg ttttttgttt tctttttaaa
     1501 tgaaaaactt tattttttaa gaggagtttc aggttcatag caaaattgag aggaaggtac
     1561 attcaagctg aggaagtttt cctctattcc tagtttactg agagattgca tcatgaatgg
     1621 gtgttaaatt ttgtcaaatg ctttttctgt gtctatcaat atgaccatgt gattttcttc
     1681 tttaacctgt tgatgggaca aattacgtta attgattttc aaacgttgaa ccacccttac
     1741 atatctggaa taaattctac ttggttgtgg tgtatatttt ttgatacatt cttggattct
     1801 ttttgctaat attttgttga aaatgtttgt atctttgttc atgagagata ttggtctgtt
     1861 gttttctttt cttgtaatgt cattttctag ttccggtatt aaggtaatgc tggcctagtt
     1921 gaatgattta ggaagtattc cctctgcttc tgtcttctga aagagattgt agaaagttga
     1981 tacaattttt ttttctttaa atatttgata gaattctgca gggcctgaaa taacctctga
     2041 aagaggaact tggttaggta cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2281 cattctccgc cccatggctg actaattttt tttatttata cagaggccga ggccgcctcg
     2341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2401 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     2461 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa atcatgaaga
     2521 ccttcaacat ctctcagcag gatctggagc tggtggaggt cgccactgag aagatcacca
     2581 tgctctatga ggacaacaag caccatgtcg gggcggccat caggaccaag actggggaga
     2641 tcatctctgc tgtccacatt gaggcctaca ttggcagggt cactgtctgt gctgaagcca
     2701 ttgccattgg gtctgctgtg agcaacgggc agaaggactt tgacaccatt gtggctgtca
     2761 ggcaccccta ctctgatgag gtggacagat ccatcagggt ggtcagcccc tgtggcatgt
     2821 gcagagagct catctctgac tatgctcctg actgctttgt gctcattgag atgaatggca
     2881 agctggtcaa aaccaccatt gaggaactca tccccctcaa gtacaccagg aactaaacct
     2941 gaattcgcta gagggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3001 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3061 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3121 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3181 tgtggtaaaa tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc
     3241 ccttcccaac agttgcaggt ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg
     3301 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     3361 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     3421 cttttcccga gggtggggga gaaccgtata taagtgcagt agtcgctgtg aacgttcttt
     3481 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     3541 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     3601 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     3661 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3721 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3781 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3841 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3901 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3961 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     4021 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     4081 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     4141 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     4201 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     4261 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     4321 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     4381 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     4441 catttcaggt gtcgtgagaa ttctgtagag atccctcgac ctcgagatcc attgtgctgg
     4501 atctgcgatc taagtaagct tcgaattctg cagtcgagct accggtcgcc accatggcct
     4561 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     4621 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4681 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     4741 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca
     4801 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4861 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctccttcatc tacaaggtga
     4921 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct
     4981 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca
     5041 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg
     5101 ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct
     5161 cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc cgccaccacc
     5221 tgttcctgag atctcgacac tagtggatcc cccgggctgc aggaattcga tatcaagctt
     5281 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5761 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5821 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     5881 taccgtcggc ccactgctcc ctaaacctga gctagcatta tccctaatac ctgccacccc
     5941 actcttaatc agtggtggaa gaacggtctc agaactgttt gtttcaattg gccatttaag
     6001 tttagtagta aaagactggt taatgataac aatgcatcgt aaaaccttca gaaggaaagg
     6061 agaatgtttt gtggaccact ttggttttct tttttgcgtg tggcagtttt aagttattag
     6121 tttttaaaat cagtactttt taatggaaac aacttgacca aaaatttgtc acagaatttt
     6181 gagacccatt aaaaaagtta aatgagaaac ctgtgtgttc ctttggtcaa caccgagaca
     6241 tttaggtgaa agacatctaa ttctggtttt acgaatctgg aaacttcttg aaaatgtaat
     6301 tcttgagtta acacttctgg gtggagaata gggttgtttt ccccccacat aattggaagg
     6361 ggaaggaata tcatttaaag ctatgggagg gtttctttga ttacaacact ggagagaaat
     6421 gcagcatgtt gctgattgcc tgtcactaaa acaggccaaa aactgagtcc ttgggttgca
     6481 tagaaagctt catgttgcta aaccaatgtt aagtgaatct ttggaaacaa aatgtttcca
     6541 aattactggg atgtgcatgt tgaaacgtgg gttaattaac tagccatgac caaaatccct
     6601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt cttaattaaa
     7321 tttttcaaaa gtagttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7381 ctcactatag gagggccatc atggccaagt tgaccagtgc tgtcccagtg ctcacagcca
     7441 gggatgtggc tggagctgtt gagttctgga ctgacaggtt ggggttctcc agagattttg
     7501 tggaggatga ctttgcaggt gtggtcagag atgatgtcac cctgttcatc tcagcagtcc
     7561 aggaccaggt ggtgcctgac aacaccctgg cttgggtgtg ggtgagagga ctggatgagc
     7621 tgtatgctga gtggagtgag gtggtctcca ccaacttcag ggatgccagt ggccctgcca
     7681 tgacagagat tggagagcag ccctggggga gagagtttgc cctgagagac ccagcaggca
     7741 actgtgtgca ctttgtggca gaggagcagg actgaggata agaattgtaa caaaaaaccc
     7801 cgccccggcg gggttttttg ttaattaa
//
