LOCUS       sequence-70658-e        5787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRed plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Johnson KM, Kines RC, Roberts JN, Lowy DR, Schiller JT, Day PM
  TITLE     Role of heparan sulfate in attachment to and infection of the murine
            female genital tract by human papillomavirus.
  JOURNAL   J Virol. 2009 Mar;83(5):2067-74. Epub 2008 Dec 10.
  PUBMED    19073722
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2009-03"; volume: "83(5)"; pages: "2067-74. Epub 2008 Dec 10"
COMMENT     Sequence Label: pRwB
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..45)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        30..359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      210..345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        407..454
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     polyA_signal    934..1055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(945..964)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     999..1018
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1238..2416
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1469..2407
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2364..2384
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2507..2516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2513..3187
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2643..2661)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2693..2713)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2911..2930
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3105..3128
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3246..3834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3299..3319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3717..3728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3876..4448
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      4619..5207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5108..5127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5293..5340
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5332..5350
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcatca agcttggtac ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
       61 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      241 attctccgcc ccatggctga ctaatttttt ttatttatac agaggccgag gccgcctcgg
      301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      361 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
      421 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa tcatgaagac
      481 cttcaacatc tctcagcagg atctggagct ggtggaggtc gccactgaga agatcaccat
      541 gctctatgag gacaacaagc accatgtcgg ggcggccatc aggaccaaga ctggggagat
      601 catctctgct gtccacattg aggcctacat tggcagggtc actgtctgtg ctgaagccat
      661 tgccattggg tctgctgtga gcaacgggca gaaggacttt gacaccattg tggctgtcag
      721 gcacccctac tctgatgagg tggacagatc catcagggtg gtcagcccct gtggcatgtg
      781 cagagagctc atctctgact atgctcctga ctgctttgtg ctcattgaga tgaatggcaa
      841 gctggtcaaa accaccattg aggaactcat ccccctcaag tacaccagga actaaacctg
      901 aattcgctag agggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
      961 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1021 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1081 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     1141 gtggtaaaat cgataaggat ccgggctggc gtaatagcga agaggcccgc accgatcgcc
     1201 cttcccaaca gttgcaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc
     1261 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     1321 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgccc
     1381 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgctgtga acgttctttt
     1441 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     1501 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     1561 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     1621 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1681 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1741 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1801 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1861 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1921 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1981 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     2041 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     2101 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     2161 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     2221 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     2281 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     2341 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     2401 atttcaggtg tcgtgagaat tctgtagaga tccctcgacc tcgagatcca ttgtgctgga
     2461 tctgcgatct aagtaagctt cgaattctgc agtcgagcta ccggtcgcca ccatggcctc
     2521 ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
     2581 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2641 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     2701 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
     2761 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2821 cgtggtgacc gtgacccagg actcctccct gcaggacggc tccttcatct acaaggtgaa
     2881 gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctatgggctg
     2941 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa
     3001 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc
     3061 caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc
     3121 ccacaacgag gactacacca tcgtggagca gtacgagcgc gccgagggcc gccaccacct
     3181 gttcctgaga tctcgacact agtggatccc ccgggctgca ggaattcgat atcaagctta
     3241 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3661 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3841 accgtcggcc cactgctccc taaacctgag ctagcattat ccctaatacc tgccacccca
     3901 ctcttaatca gtggtggaag aacggtctca gaactgtttg tttcaattgg ccatttaagt
     3961 ttagtagtaa aagactggtt aatgataaca atgcatcgta aaaccttcag aaggaaagga
     4021 gaatgttttg tggaccactt tggttttctt ttttgcgtgt ggcagtttta agttattagt
     4081 ttttaaaatc agtacttttt aatggaaaca acttgaccaa aaatttgtca cagaattttg
     4141 agacccatta aaaaagttaa atgagaaacc tgtgtgttcc tttggtcaac accgagacat
     4201 ttaggtgaaa gacatctaat tctggtttta cgaatctgga aacttcttga aaatgtaatt
     4261 cttgagttaa cacttctggg tggagaatag ggttgttttc cccccacata attggaaggg
     4321 gaaggaatat catttaaagc tatgggaggg tttctttgat tacaacactg gagagaaatg
     4381 cagcatgttg ctgattgcct gtcactaaaa caggccaaaa actgagtcct tgggttgcat
     4441 agaaagcttc atgttgctaa accaatgtta agtgaatctt tggaaacaaa atgtttccaa
     4501 attactggga tgtgcatgtt gaaacgtggg ttaattaact agccatgacc aaaatccctt
     4561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc ttaattaaat
     5281 ttttcaaaag tagttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     5341 tcactatagg agggccatca tggccaagtt gaccagtgct gtcccagtgc tcacagccag
     5401 ggatgtggct ggagctgttg agttctggac tgacaggttg gggttctcca gagattttgt
     5461 ggaggatgac tttgcaggtg tggtcagaga tgatgtcacc ctgttcatct cagcagtcca
     5521 ggaccaggtg gtgcctgaca acaccctggc ttgggtgtgg gtgagaggac tggatgagct
     5581 gtatgctgag tggagtgagg tggtctccac caacttcagg gatgccagtg gccctgccat
     5641 gacagagatt ggagagcagc cctgggggag agagtttgcc ctgagagacc cagcaggcaa
     5701 ctgtgtgcac tttgtggcag aggagcagga ctgaggataa gaattgtaac aaaaaacccc
     5761 gccccggcgg ggttttttgt taattaa
//
