LOCUS       sequence-7066-ex        1433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1433)
  TITLE     Salvesen Casp1, Casp2 plasmids
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Casp2-Flag
FEATURES             Location/Qualifiers
     source          1..1433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1397..1420
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atacgactca ttatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc
       61 cgccagtgtg ctggaattcg ccaccatggc cgctgacagg ggacgcagga tattgggagt
      121 gtgtggcatg catcctcatc atcaggaaac tctaaaaaag aaccgagtgg tgctagccaa
      181 acagctgttg ttgagcgaat tgttagaaca tcttctggag aaggacatca tcaccttgga
      241 aatgagggag ctcatccagg ccaaagtggg cagtttcagc cagaatgtgg aactcctcaa
      301 cttgctgcct aagaggggtc cccaagcttt tgatgccttc tgtgaagcac tgagggagac
      361 caagcaaggc cacctggagg atatgttgct caccaccctt tctgggcttc agcatgtact
      421 cccaccgttg agctgtgact acgacttgag tctccctttt ccggtgtgtg agtcctgtcc
      481 cctttacaag aagctccgcc tgtcgacaga tactgtggaa cactccctag acaataaaga
      541 tggtcctgtc tgccttcagg tgaagccttg cactcctgaa ttttatcaaa cacacttcca
      601 gctggcatat aggttgcagt ctcggcctcg tggcctagca ctggtgttga gcaatgtgca
      661 cttcactgga gagaaagaac tggaatttcg ctctggaggg gatgtggacc acagtactct
      721 agtcaccctc ttcaagcttt tgggctatga cgtccatgtt ctatgtgacc agactgcaca
      781 ggaaatgcaa gagaaactgc agaattttgc acagttacct gcacaccgag tcacggactc
      841 ctgcatcgtg gcactcctct cgcatggtgt ggagggcgcc atctatggtg tggatgggaa
      901 actgctccag ctccaagagg tttttcagct ctttgacaac gccaactgcc caagcctaca
      961 gaacaaacca aaaatgttct tcatccaggc ctgccgtgga gatgagactg atcgtggggt
     1021 tgaccaacaa gatggaaaga accacgcagg atcccctggg tgcgaggaga gtgatgccgg
     1081 taaagaaaag ttgccgaaga tgagactgcc cacgcgctca gacatgatat gcggctatgc
     1141 ctgcctcaaa gggactgccg ccatgcggaa caccaaacga ggttcctggt acatcgaggc
     1201 tcttgctcaa gtgttttctg agcgggcttg tgatatgcac gtggccgaca tgctggttaa
     1261 ggtgaacgca cttatcaagg atcgggaagg ttatgctcct ggcacagaat tccaccggtg
     1321 caaggagatg tctgaatact gcagcactct gtgccgccac ctctacctgt tcccaggaca
     1381 ccctcccaca ctcgaggact acaaggacga cgatgacaag tgatctagag ggc
//
