LOCUS       sequence-70663-e        6519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tandem dual tomato plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: ptwB
FEATURES             Location/Qualifiers
     source          1..6519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..73)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        58..387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      238..373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        435..482
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     polyA_signal    962..1083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(973..992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1027..1046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1265..2443
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1496..2434
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2391..2411
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(2484..2492,2493..2507)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      2514..2523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2520..3950
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    3978..4566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4031..4051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4449..4460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4608..5180
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      5351..5939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5840..5859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6025..6072
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6064..6082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacct gtggaatgtg
       61 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      301 atttatacag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      361 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
      421 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
      481 acaaggtgag gaactaaatc atgaagacct tcaacatctc tcagcaggat ctggagctgg
      541 tggaggtcgc cactgagaag atcaccatgc tctatgagga caacaagcac catgtcgggg
      601 cggccatcag gaccaagact ggggagatca tctctgctgt ccacattgag gcctacattg
      661 gcagggtcac tgtctgtgct gaagccattg ccattgggtc tgctgtgagc aacgggcaga
      721 aggactttga caccattgtg gctgtcaggc acccctactc tgatgaggtg gacagatcca
      781 tcagggtggt cagcccctgt ggcatgtgca gagagctcat ctctgactat gctcctgact
      841 gctttgtgct cattgagatg aatggcaagc tggtcaaaac caccattgag gaactcatcc
      901 ccctcaagta caccaggaac taaacctgaa ttcgctagag ggccgcttcg agcagacatg
      961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1081 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1141 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt
     1201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcggtggag aagagcatgc
     1261 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1321 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     1381 aagtgatgtc gtgtactggc tccgcccttt tcccgagggt gggggagaac cgtatataag
     1441 tgcagtagtc gctgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1501 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1561 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1621 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1681 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1741 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1801 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1861 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1921 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1981 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2041 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2101 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2161 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2221 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2281 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2341 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2401 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tctagcatga
     2461 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat cccgccacca
     2521 tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     2581 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     2641 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     2701 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     2761 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     2821 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     2881 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     2941 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     3001 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
     3061 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg
     3121 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     3181 gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg
     3241 cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc aaggtgcgca
     3301 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3361 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3421 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     3481 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3541 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     3601 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     3661 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     3721 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     3781 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     3841 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     3901 agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa gaattcgaag
     3961 ctatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4381 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4561 ccccgcatcg ataccgtcgg cccactgctc cctaaacctg agctagcatt atccctaata
     4621 cctgccaccc cactcttaat cagtggtgga agaacggtct cagaactgtt tgtttcaatt
     4681 ggccatttaa gtttagtagt aaaagactgg ttaatgataa caatgcatcg taaaaccttc
     4741 agaaggaaag gagaatgttt tgtggaccac tttggttttc ttttttgcgt gtggcagttt
     4801 taagttatta gtttttaaaa tcagtacttt ttaatggaaa caacttgacc aaaaatttgt
     4861 cacagaattt tgagacccat taaaaaagtt aaatgagaaa cctgtgtgtt cctttggtca
     4921 acaccgagac atttaggtga aagacatcta attctggttt tacgaatctg gaaacttctt
     4981 gaaaatgtaa ttcttgagtt aacacttctg ggtggagaat agggttgttt tccccccaca
     5041 taattggaag gggaaggaat atcatttaaa gctatgggag ggtttctttg attacaacac
     5101 tggagagaaa tgcagcatgt tgctgattgc ctgtcactaa aacaggccaa aaactgagtc
     5161 cttgggttgc atagaaagct tcatgttgct aaaccaatgt taagtgaatc tttggaaaca
     5221 aaatgtttcc aaattactgg gatgtgcatg ttgaaacgtg ggttaattaa ctagccatga
     5281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6001 tcttaattaa atttttcaaa agtagttgac aattaatcat cggcatagta tatcggcata
     6061 gtataatacg actcactata ggagggccat catggccaag ttgaccagtg ctgtcccagt
     6121 gctcacagcc agggatgtgg ctggagctgt tgagttctgg actgacaggt tggggttctc
     6181 cagagatttt gtggaggatg actttgcagg tgtggtcaga gatgatgtca ccctgttcat
     6241 ctcagcagtc caggaccagg tggtgcctga caacaccctg gcttgggtgt gggtgagagg
     6301 actggatgag ctgtatgctg agtggagtga ggtggtctcc accaacttca gggatgccag
     6361 tggccctgcc atgacagaga ttggagagca gccctggggg agagagtttg ccctgagaga
     6421 cccagcaggc aactgtgtgc actttgtggc agaggagcag gactgaggat aagaattgta
     6481 acaaaaaacc ccgccccggc ggggtttttt gttaattaa
//
