LOCUS       sequence-70667-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     Ralf Kuehn TALEN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-RabZFN-B
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tacatggttc atgccttctt ctttttccta cagatcctta attaataata cgactcacta
       61 taggggccgc caccatgaga tctcctaaga aaaagaggaa ggtggcggcc gctgactaca
      121 aggatgacga cgataaacca tggggtggcg gaggtagtcc attccagtgt agaatctgta
      181 tgcgaaactt ttcaacgtct gggcacttgt ccaggcacat aaggacacac accggagaga
      241 aacccttcgc ttgcgacata tgtggaagga agttcgccca taaatggcag aggaacaagc
      301 ataccaagat tcacacaggg gaaaaacctt tccagtgtcg gatttgtatg cgaaatttct
      361 cagaccgctc agttctacgg agacacattc gtacccatac cggcgaaaag ccgtttgcat
      421 gcgacatctg tggcagaaaa tttgctgact ccagcactcg caagaagcac acgaaaattc
      481 acaccggacg ccagaaaccc tttcagtgtc gaatttgcat gcgtaacttt tctcgatccg
      541 accatctgtc ggaacacatc cgaacccata ctggcgaaaa gccattcgcg tgcgacatct
      601 gtggtagaaa gttcgccgat aagtctaata ggaagaagca cacgaaaatt cacctgcgcg
      661 gatcccagct cgtgaaaagc gaactcgaag aaaagaaaag tgaactgcgg cacaaactga
      721 aatacgtccc acatgaatac attgagctga tcgagattgc taggaactcc acccaggaca
      781 gaatcctcga gatgaaagtg atggaattct ttatgaaa
//
