LOCUS       sequence-7067-ex        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  TITLE     Salvesen Casp1, Casp2 plasmids
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Casp2 C303A-Flag
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1388..1411
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1430..1448)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1431..1448)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttatagggag gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
       61 gctggaattc gccaccatgg ccgctgacag gggacgcagg atattgggag tgtgtggcat
      121 gcatcctcat catcaggaaa ctctaaaaaa gaaccgagtg gtgctagcca aacagctgtt
      181 gttgagcgaa ttgttagaac atcttctgga gaaggacatc atcaccttgg aaatgaggga
      241 gctcatccag gccaaagtgg gcagtttcag ccagaatgtg gaactcctca acttgctgcc
      301 taagaggggt ccccaagctt ttgatgcctt ctgtgaagca ctgagggaga ccaagcaagg
      361 ccacctggag gatatgttgc tcaccaccct ttctgggctt cagcatgtac tcccaccgtt
      421 gagctgtgac tacgacttga gtctcccttt tccggtgtgt gagtcctgtc ccctttacaa
      481 gaagctccgc ctgtcgacag atactgtgga acactcccta gacaataaag atggtcctgt
      541 ctgccttcag gtgaagcctt gcactcctga attttatcaa acacacttcc agctggcata
      601 taggttgcag tctcggcctc gtggcctagc actggtgttg agcaatgtgc acttcactgg
      661 agagaaagaa ctggaatttc gctctggagg ggatgtggac cacagtactc tagtcaccct
      721 cttcaagctt ttgggctatg acgtccatgt tctatgtgac cagactgcac aggaaatgca
      781 agagaaactg cagaattttg cacagttacc tgcacaccga gtcacggact cctgcatcgt
      841 ggcactcctc tcgcatggtg tggagggcgc catctatggt gtggatggga aactgctcca
      901 gctccaagag gtttttcagc tctttgacaa cgccaactgc ccaagcctac agaacaaacc
      961 aaaaatgttc ttcatccagg ccgcccgtgg agatgagact gatcgtgggg ttgaccaaca
     1021 agatggaaag aaccacgcag gatcccctgg gtgcgaggag agtgatgccg gtaaagaaaa
     1081 gttgccgaag atgagactgc ccacgcgctc agacatgata tgcggctatg cctgcctcaa
     1141 agggactgcc gccatgcgga acaccaaacg aggttcctgg tacatcgagg ctcttgctca
     1201 agtgttttct gagcgggctt gtgatatgca cgtggccgac atgctggtta aggtgaacgc
     1261 acttatcaag gatcgggaag gttatgctcc tggcacagaa ttccaccggt gcaaggagat
     1321 gtctgaatac tgcagcactc tgtgccgcca cctctacctg ttcccaggac accctcccac
     1381 actcgaggac tacaaggacg acgatgacaa gtgatctaga gggccctatt ctatagtgtc
     1441 acctaaatg
//
