LOCUS       sequence-70683-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for I-Sce I in a 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (Sce)
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..212)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
       61 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
      121 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
      181 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctctgcagct
      241 agaatcagtc cggatcactc ttccatcacg ccgatctgcc aggccagaac ctgggcgact
      301 agctcgcagg cttggcggcc atcgagatcg acgtagtacg gcgttcggtg atggcaaacg
      361 atcaatcccc acagtcgctc gccgcgcaaa atcgagatcg acatcgtgcc gtgcatgcct
      421 atgttgcgca tgaattccag atggacgggc gagacgctgc gcaggatggc gaagctaaga
      481 tcaatcggcc gcccggtgac cggattgagg tctggggtga ccggcaccgg ccgataattg
      541 atatcgggaa tgatccgtac cgggttgatg gtatagagcc gacgggcctg cgccggcacg
      601 gttgaggcag gatagtgcag gcctagtttt gactcgacct cggcgcaccg atcctctgcg
      661 atcacttcgc cgctgaagtc ggaggcgaag cgatagatca tcacccgatc gaagccggta
      721 atcttccgca cctcttgcgc cgcggcggcg caggcgcttt ccaaggtttc ggcggcctgc
      781 aggcggcgga tggcgctgtt ggtgcggcgg aagaacgcct gcggctcggc gacgtcccgc
      841 tggggaggct cgagctcgag gaagatgagc tgatcatggc gatgccagga gccgatgaag
      901 cctgcgtcct ttcgcatcgt gaagccgaca gtgatcggtg ctccgacggc cgccccgggc
      961 tcggccaagg cgatcgtcag acggttgtgc gtctccgagt cgaagacatc ggccgcagag
     1021 cggccgatca gggcgccgat cgccagtccg gtgagttcgg gaaggttgtc gctgccggca
     1081 a
//
