LOCUS       sequence-70706-e        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MBP myocilin olfactomedin domain fusion protein with a TEV
            protease recognition sequence and the mutation I499F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Burns JN, Turnage KC, Walker CA, Lieberman RL
  TITLE     The stability of myocilin olfactomedin domain variants provides new 
            insight into glaucoma as a protein misfolding disorder.
  JOURNAL   Biochemistry. 2011 Jul 5;50(26):5824-33. doi: 10.1021/bi200231x. 
            Epub 2011 Jun 9.
  PUBMED    21612213
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi200231x";
            journalName: "Biochemistry"; date: "2011-07-5- 5"; volume: "50"; 
            issue: "26"; pages: "5824-33"
COMMENT     Sequence Label: pmalc4x_I499F_TEV
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(888..908)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(918..941)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 cttgcctgca ggtcgactct agaggatccg aattcaggag agttagagcc ttatcacatc
       61 ttggaaagct tgaagtcata agtgaccatg ttcaagttgt cccaggcaaa gagcttcttc
      121 tccagggggt tgtagtcaat catgctgctg tacttatagc ggttcttgaa tgggatggtc
      181 agggtcttgc tgatacctgt gcctgtgtca taagcaaagt tgacggtagc atctgctgag
      241 gtgtagctgc tgacggtgta caaggtgcca cagatgatga aggcattggc gactgactgc
      301 ttacggatgt ttgtctccca ggtttgttcg agttccagat tctctgggtt cagtttggag
      361 aggacaatgg cacctttggc ctcatcggtg ctgtaaatga cccagaggcc tgcttcatcc
      421 acagccaagt caatgtccgt gtagccaccc caagaatacg ggaactgtcc gtggtagcca
      481 gctccaggga tttccttctc agccttcact gtctcggtat tcagctcata tcttatgaca
      541 gttctggact cagcgccctg gaaatagagg ctccccgagt acaccacagc acccgtgctt
      601 tccagtggcc taggcagtat gtgaacctta gaagggtagc cctgcataaa ctggctgatg
      661 aggtcatact caaaaacctg gcggacatcc gtgccaactg tgtcgattct ccacgtggtc
      721 tcctgggtgt aggggtaggt gggcttgggg tctcgcatcc acacaccata cttgccagta
      781 attgtttctg ctgttctcag cgtgagaggc tctcctaccc aaactagttc tccacatccg
      841 gtgtctccct ctccactcct gagatagcca gatgggctct ccttcaagct ctgaaaataa
      901 agattctccg aattagtctg cgcgtctttc agggcttcat cgacagtctg acgaccgctg
      961 gcggcgttga tcaccgcagt acgcacggca taccagaaag cggacatctg cgggatgttc
     1021 ggcatgattt cacctttctg ggcgttttcc atagtggcgg caatccgcgg atctttcacc
     1081 aactcttcct cgta
//
