LOCUS       sequence-70708-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA4.full length FVIII D1241E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Peters lab - FVIII constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FVIII H2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gttaagcttc gcgacgtacg gccgccacca tgcaaataga gctctccacc
      121 tgcttctttc tgtgcctttt gcgattctgc tttagtgcca ccagaagata ctacctgggt
      181 gcagtggaac tgtcatggga ctatatgcaa agtgatctcg gtgagctgcc tgtggacgca
      241 agatttcctc ctagagtgcc aaaatctttt ccattcaaca cctcagtcgt gtacaaaaag
      301 actctgtttg tagaattcac ggatcacctt ttcaacatcg ctaagccaag gccaccctgg
      361 atgggtctgc taggtcctac catccaggct gaggtttatg atacagtggt cattacactt
      421 aagaacatgg cttcccatcc tgtcagtctt catgctgttg gtgtatccta ctggaaagct
      481 tctgagggag ctgaatatga tgatcagacc agtcaaaggg agaaagaaga tgataaagtc
      541 ttccctggtg gaagccatac atatgtctgg caggtcctga aagagaatgg tccaatggcc
      601 tctgacccac tgtgccttac ctactcatat ctttctcatg tggacctggt aaaagacttg
      661 aattcaggcc tcattggagc cctactagta tgtagagaag ggagtctggc caaggaaaag
      721 acacagacct tgcacaaatt tatactactt tttgctgtat ttgatgaagg gaaaagttgg
      781 cactcagaaa caaagaactc cttgatgcag gatagggatg ctgcatctgc tcgggcctgg
      841 cctaaaatgc acacagtcaa tggttatgta aacaggtctc tgccaggtct gattggatg
//
