LOCUS       sequence-70715-e        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses engineered halorhodopsin 3.0 (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(149..182)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcataact tcgtatagca tacattatac gaagttattc tttgcaccat tctaaagaat
      121 aacagtgata atttctgggt taaggcaaat aacttcgtat aggatacttt atacgaagtt
      181 atcgctagct ctagagcggt accggatccc cgggtaccgg taggccacca tgacagagac
      241 cctgcctccc gtgaccgaga gtgccgtggc ccttcaagcc gaggttaccc aaagggagtt
      301 gttcgagttc gtgctgaacg accctttgct tgcaagcagt ctctatatca acatcgcact
      361 tgcaggactg agtatactgc tgttcgtttt tatgacccga ggactcgatg atccacgggc
      421 aaaacttatt gctgtgtcaa ccatccttgt gcctgtcgtc agcattgcct cctacactgg
      481 attggcgagc ggcctgacaa tttccgttct tgaaatgcca gcgggccatt ttgcagaagg
      541 cagctcagtg atgctgggag gagaagaggt agatggtgta gtcaccatgt ggggacggta
      601 tctcacctgg gcactttcca cgcccatgat tctcctcgct ctgggtctcc tggccggaag
      661 caatgctaca aagctcttca cagctatcac tttcgatatc gctatgtgcg tgactggcct
      721 tgccgcggcc ctgactacct cctcccacct catgagatgg ttctggtacg ctatcagttg
      781 tgcatgcttt ctggtggtct tgtatatcct gctggtggag tgggcacagg acgccaaagc
      841 cgcgggaacc gctgacatgt tcaataccct gaagctgttg acagtagtga tgtggctggg
      901 gtatccaatt gtgtgggctc ttggagtcga gggtatcgcg gtgttgcccg ttggggtgac
      961 gagctgggga tattctttcc tggatatcgt ggcaaagtac attttcgcat tcttgctcct
     1021 gaactatctg acgtcaaacg aatctgtcgt gtccggcagc attttgg
//
