LOCUS       sequence-70717-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tagged Miro2 A13V constitutively active mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Fransson S, Ruusala A, Aspenstrom P
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in 
            mitochondrial trafficking.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 
            3.
  PUBMED    16630562
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-06-2"; volume: "344(2)"; pages: 
            "500-10. Epub 2006 Apr 3"
COMMENT     Sequence Label: pRK5-myc-Miro2 A13V
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..104
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     86..103
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     117..136
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      183..192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..221
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cctatagagt ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca
       61 taaccttatg tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc
      121 ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat
      181 tggccaccat ggagcagaag ctgatctccg aggaggacct gggatctatg aggcgggacg
      241 tgcgcatcct gttactgggc gaggtccagg tggggaagac gtcgctgatc ctgtccctgg
      301 tgggcgagga gttccccgag gaggtccctc cccgcgcgga ggagatcacc atccccgcgg
      361 acgtcacccc ggagaaggtg cccacccaca tcgtggacta ctcagaagcc gagcagacgg
      421 acgaggagct gcgggaggag atccacaagg caaacgtggt gtgtgtggtg tatgacgtct
      481 ctgaggaggc caccattgag aagattcgaa ctaagtggat cccactggtg aatgggggga
      541 ccacgcaggg gcccagggtg cccatcatcc tagtgggcaa caagtcagac ctgcggtcgg
      601 ggagctccat ggaggccgtg ctccccatca tgagccagtt ttccgagatt gagacctgcg
      661 tggagtgttc ggccaagaac ctgaggaaca tctcagagct gttctactac gcc
//
