LOCUS       sequence-70718-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tagged Miro2 T18N dominant negative mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Fransson S, Ruusala A, Aspenstrom P
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in 
            mitochondrial trafficking.
  JOURNAL   Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 
            3.
  PUBMED    16630562
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2006-06-2"; volume: "344(2)"; pages: 
            "500-10. Epub 2006 Apr 3"
COMMENT     Sequence Label: pRK5-myc-Miro2 T18N
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        167..185
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     167..184
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     198..217
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             273..302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgntg
       61 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg gcttcgttag
      121 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
      181 atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
      241 cctcggttct atcgattgaa ttggccacca tggagcagaa gctgatctcc gaggaggacc
      301 tgggatctat gaggcgggac gtgcgcatcc tgttactggg cgaggcccag gtggggaaga
      361 actcgctgat cctgtccctg gtgggcgagg agttccccga ggaggtccct ccccgcgcgg
      421 aggagatcac catccccgcg gacgtcaccc cggagaaggt gcccacccac atcgtggact
      481 actcagaagc cgagcagacg gacgaggagc tgcgggagga gatccacaag gcaaacgtgg
      541 tgtgtgtggt gtatgacgtc tctgaggagg ccaccattga gaagattcga actaagtgga
      601 tcccactggt gaatgggggg accacgcagg ggcccagggt gcccatcatc ctagtgggca
      661 acaagtcaga cctgcggtcg gggagctcca tggaggccgt gctccccatc atgagccagt
      721 tttccgagat tgagacctgc gtggagtgtt cggccaagaa cctgaggaac atctcagagc
      781 tgttctacta cgcccag
//
