LOCUS       sequence-70719-e        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses engineered halorhodopsin 3.0 (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(132..165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catttcaggt gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata
       61 ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat
      121 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gccataagct
      181 tgatatcgaa ttctcattac acctcgttct cgtagcagaa cttgtacagc tcttccatca
      241 cgccgatctg ccaggccaga acctgggcga ctagctcgca ggcttggcgg ccatcgagat
      301 cgacgtagta cggcgttcgg tgatggcaaa cgatcaatcc ccacagtcgc tcgccgcgca
      361 aaatcgagat cgacatcgtg ccgtgcatgc ctatgttgcg catgaattcc agatggacgg
      421 gcgagacgct gcgcaggatg gcgaagctaa gatcaatcgg ccgcccggtg accggattga
      481 ggtctggggt gaccggcacc ggccgataat tgatatcggg aatgatccgt accgggttga
      541 tggtatagag ccgacgggcc tgcgccggca cggttgaggc aggatagtgc aggcctagtt
      601 ttgactcgac ctcggcgcac cgatcctctg cgatcacttc gccgctgaag tcggaggcga
      661 agcgatagat catcacccga tcgaagccgg taatcttccg cacctcttgc gccgcggcgg
      721 cgcaggcgct ttccaaggtt tcggcggcct gcaggcggcg gatggcgctg ttggtgcggc
      781 ggaagaacgc ctgcggctcg gcgacgtccc gctggggagg ctcgagctcg aggaagatga
      841 gctgatcatg gcgatgccag gagccgatga agcctgcgtc ctttcgcatc gtgaagccga
      901 cagtgatcgg tgctccgacg gccgccccgg gctcggccaa ggcgatcgtc agacggttgt
      961 gcgtctccga gtcgaagaca tcggccgcag agcggccgat cagggcgccg atcgccagtc
     1021 cggtgagttc gggaaggttg tcgctgccgg caacgatcgt catgtcg
//
