LOCUS       sequence-70723-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses engineered halorhodopsin 3.0 (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1028..1061
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ggctgcctgg cgacggatcc ttcagccata ctagtcacca cgttgatgtc gatctggtcc
       61 agggggatgt actcgccctc gctggtgatc ctgctcttgg cggccgcatc atcagccggg
      121 gtcccagaag cagatggaac atccaaaatg ctgccggaca cgacagattc gtttgacgtc
      181 agatagttca ggagcaagaa tgcgaaaatg tactttgcca cgatatccag gaaagaatat
      241 ccccagctcg tcaccccaac gggcaacacc gcgataccct cgactccaag agcccacaca
      301 attggatacc ccagccacat cactactgtc aacagcttca gggtattgaa catgtcagcg
      361 gttcccgcgg ctttggcgtc ctgtgcccac tccaccagca ggatatacaa gaccaccaga
      421 aagcatgcac aactgatagc gtaccagaac catctcatga ggtgggagga ggtagtcagg
      481 gccgcggcaa ggccagtcac gcacatagcg atatcgaaag tgatagctgt gaagagcttt
      541 gtagcattgc ttccggccag gagacccaga gcgaggagaa tcatgggcgt ggaaagtgcc
      601 caggtgagat accgtcccca catggtgact acaccatcta cctcttctcc tcccagcatc
      661 actgagctgc cttctgcaaa atggcccgct ggcatttcaa gaacggaaat tgtcaggccg
      721 ctcgccaatc cagtgtagga ggcaatgctg acgacaggca caaggatggt tgacacagca
      781 ataagttttg cccgtggatc atcgagtcct cgggtcataa aaacgaacag cagtatactc
      841 agtcctgcaa gtgcgatgtt gatatagaga ctgcttgcaa gcaaagggtc gttcagcacg
      901 aactcgaaca actccctttg ggtaacctcg gcttgaaggg ccacggcact ctcggtcacg
      961 ggaggcaggg tctctgtcat ggtggcctac cggtacccgg ggatccggta ccgctctaga
     1021 gctagcgata acttcgtata aagtatccta tacgaagtta tttgccttaa ccc
//
