LOCUS       sequence-70724-e        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, 
            double floxed inverted open reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC374 - pAAV EF1a DIO iRFP
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(149..182)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcataact tcgtatagca tacattatac gaagttattc tttgcaccat tctaaagaat
      121 aacagtgata atttctgggt taaggcaaat aacttcgtat aggatacttt atacgaagtt
      181 atcgctagcg ctaccggact cagatctgcc gccaccatgg ctgaaggatc cgtcgccagg
      241 cagcctgacc tcttgacctg cgacgatgag ccgatccata tccccggtgc catccaaccg
      301 catggactgc tgctcgccct cgccgccgac atgacgatcg ttgccggcag cgacaacctt
      361 cccgaactca ccggactggc gatcggcgcc ctgatcggcc gctctgcggc cgatgtcttc
      421 gactcggaga cgcacaaccg tctgacgatc gccttggccg agcccggggc ggccgtcgga
      481 gcaccgatca ctgtcggctt cacgatgcga aaggacgcag gcttcatcgg ctcctggcat
      541 cgccatgatc agctcatctt cctcgagctc gagcctcccc agcgggacgt cgccgagccg
      601 caggcgttct tccgccgcac caacagcgcc atccgccgcc tgcaggccgc cgaaaccttg
      661 gaaagcgcct gcgccgccgc ggcgcaagag gtgcggaaga ttaccggctt cgatcgggtg
      721 atgatctatc gcttcgcctc cgacttcagc ggcgaagtga tcgcagagga tcggtgcgcc
      781 gaggtcgagt caaaactagg cctgcactat cctgcctcaa ccgtgccggc gcaggcccgt
      841 cggctctata ccatcaaccc ggtacggatc attcccgata tcaattatcg gccggtgccg
      901 gtcaccccag acctcaatcc ggtcaccggg cggccgattg atcttagctt cgccatcctg
      961 cgcagcgtct cgcccgtcca tctggaattc atgcgcaaca taggcatgca cggcacgatg
     1021 tcgatctcga ttttgcgcgg cgagcgactg tggggattga tcgtttgcca tcaccgaacg
     1081 ccgtactacg tcgatctcga tggccgccaa gcctgcgag
//
