LOCUS       sequence-70725-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(131..164)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atttcaggtg tcgtgaggta ccggatcctc tagagtcgac tccggaataa cttcgtatag
       61 gatactttat acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg
      121 aaacctctta ataacttcgt atagcataca ttatacgaag ttatggcgcg ccataagctt
      181 gatatcgaat tcgtcgactc actcttccat cacgccgatc tgccaggcca gaacctgggc
      241 gactagctcg caggcttggc ggccatcgag atcgacgtag tacggcgttc ggtgatggca
      301 aacgatcaat ccccacagtc gctcgccgcg caaaatcgag atcgacatcg tgccgtgcat
      361 gcctatgttg cgcatgaatt ccagatggac gggcgagacg ctgcgcagga tggcgaagct
      421 aagatcaatc ggccgcccgg tgaccggatt gaggtctggg gtgaccggca ccggccgata
      481 attgatatcg ggaatgatcc gtaccgggtt gatggtatag agccgacggg cctgcgccgg
      541 cacggttgag gcaggatagt gcaggcctag ttttgactcg acctcggcgc accgatcctc
      601 tgcgatcact tcgccgctga agtcggaggc gaagcgatag atcatcaccc gatcgaagcc
      661 ggtaatcttc cgcacctctt gcgccgcggc ggcgcaggcg ctttccaagg tttcggcggc
      721 ctgcaggcgg cggatggcgc tgttggtgcg gcggaagaac gcctgcggct cggcgacgtc
      781 ccgctgggga ggctcgagct cgaggaagat gagctgatca tggcgatgcc aggagccgat
      841 gaagcctgcg tcctttcgca tcgtgaagcc gacagtgatc ggtgctccga cggccgcccc
      901 gggctcggcc aaggcgatcg tcagacggtt gtgcgtctcc gagtcgaaga catcggccgc
      961 agagcggccg atcagggcgc cgatcgccag tccggtgagt tcgggaaggt tgtcgctgcc
     1021 ggcaacgatc gtcatgtcg
//
