LOCUS       sequence-70726-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) in a Cre-dependent manner (DIO, double floxed inverted open 
            reading frame)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(149..182)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcataact tcgtatagca tacattatac gaagttattc tttgcaccat tctaaagaat
      121 aacagtgata atttctgggt taaggcaaat aacttcgtat aggatacttt atacgaagtt
      181 atcgctagct ctagagcggt accggatcct ctagagtcga ctccggagcc accatggact
      241 atggcggcgc tttgtctgcc gtcggacgcg aacttttgtt cgttactaat cctgtggtgg
      301 tgaacgggtc cgtcctggtc cctgaggatc aatgttactg tgccggatgg attgaatctc
      361 gcggcacgaa cggcgctcag accgcgtcaa atgtcctgca gtggcttgca gcaggattca
      421 gcattttgct gctgatgttc tatgcctacc aaacctggaa atctacatgc ggctgggagg
      481 agatctatgt gtgcgccatt gaaatggtta aggtgattct cgagttcttt tttgagttta
      541 agaatccctc tatgctctac cttgccacag gacaccgggt gcagtggctg cgctatgcag
      601 agtggctgct cacttgtcct gtcatcctta tccgcctgag caacctcacc ggcctgagca
      661 acgactacag caggagaacc atgggactcc ttgtctcaga catcgggact atcgtgtggg
      721 gggctaccag cgccatggca accggctatg ttaaagtcat cttcttttgt cttggattgt
      781 gctatggcgc gaacacattt tttcacgccg ccaaagcata tatcgagggt tatcatactg
      841 tgccaaaggg tcggtgccgc caggtcgtga ccggcatggc atggctgttt ttcgtgagct
      901 ggggtatgtt cccaattctc ttcattttgg ggcccgaagg ttttggcgtc ctgagcgtct
      961 atggctccac cgtaggtcac acgattattg atctgatgag taaaaa
//
